Silent scaffolds: inhibition OF c-Jun N-terminal kinase 3 activity in cell by dominant-negative arrestin-3 mutant

• Published in: The Journal of biological chemistry Vol. 287; no. 23; pp. 19653 - 19664
• Main Authors: Breitman, Maya, Kook, Seunghyi, Gimenez, Luis E, Lizama, Britney N, Palazzo, Maria C, Gurevich, Eugenia V, Gurevich, Vsevolod V
• Format: Journal Article
• Language: English
• Published: United States American Society for Biochemistry and Molecular Biology 01.06.2012
• Subjects: Animals; Arrestins - genetics; Arrestins - metabolism; Biological Assay - methods; Chlorocebus aethiops; COS Cells; Enzyme Activation - genetics; Humans; MAP Kinase Kinase 4 - genetics; MAP Kinase Kinase 4 - metabolism; MAP Kinase Kinase Kinase 5 - genetics; MAP Kinase Kinase Kinase 5 - metabolism; MAP Kinase Signaling System; Mitogen-Activated Protein Kinase 10 - genetics; Mitogen-Activated Protein Kinase 10 - metabolism; Mutation; Phosphorylation - genetics; Protein Binding - genetics; Signal Transduction

We established a new in vivo arrestin-3-JNK3 interaction assay based on bioluminescence resonance energy transfer (BRET) between JNK3-luciferase and Venus-arrestins. We tested the ability of WT arrestin-3 and its 3A mutant that readily binds β2-adrenergic receptors as well as two mutants impaired in...