Stable production of an analog of human tissue plasminogen activator from cultured Drosophila cells

• Published in: Cytotechnology (Dordrecht) Vol. 10; no. 2; p. 157
• Main Authors: Olsen, M K, Rockenbach, S K, Fischer, H D, Hoogerheide, J G, Tomich, C S
• Format: Journal Article
• Language: English
• Published: United States 1992
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Cinnamates; Culture Media, Serum-Free; Drosophila melanogaster - cytology; Drug Resistance; Gene Expression Regulation - drug effects; Genetic Vectors; Humans; Hygromycin B - analogs & derivatives; Hygromycin B - pharmacology; Metallothionein - genetics; Molecular Sequence Data; Promoter Regions, Genetic; Recombinant Fusion Proteins - biosynthesis; Recombinant Fusion Proteins - pharmacology; Tissue Plasminogen Activator - biosynthesis; Tissue Plasminogen Activator - pharmacology

We have studied the expression of an analog of human tissue plasminogen activator, FK2P, in Drosophila Schneider 2 cells. A number of promoters were tested, including the Drosophila metallothionein promoter (MTd), baculovirus immediate early promoter (IE), Drosophila copia promoter, mouse metallothi...