Unraveling ultrafast photoinduced proton transfer dynamics in a fluorescent protein biosensor for Ca(2+) imaging

• Published in: Chemistry : a European journal Vol. 21; no. 17; pp. 6481 - 6490
• Main Authors: Tang, Longteng, Liu, Weimin, Wang, Yanli, Zhao, Yongxin, Oscar, Breland G, Campbell, Robert E, Fang, Chong
• Format: Journal Article
• Language: English
• Published: Germany 20.04.2015
• Subjects: Calcium - chemistry; Green Fluorescent Proteins - chemistry; Models, Molecular; Molecular Structure; Photochemical Processes; Protons; vibrational spectroscopy; excited state proton transport; femtochemistry; calcium ion sensing; fluorescent probes

Imaging Ca(2+) dynamics in living systems holds great potential to advance neuroscience and cellular biology. G-GECO1.1 is an intensiometric fluorescent protein Ca(2+) biosensor with a Thr-Tyr-Gly chromophore. The protonated chromophore emits green upon photoexcitation via excited-state proton trans...