In vivo gene therapy for α-fetoprotein-producing hepatocellular carcinoma by adenovirus-mediated transfer of cytosine deaminase gene

• Published in: Cancer research (Chicago, Ill.) Vol. 57; no. 3; pp. 461 - 465
• Main Authors: KANAI, F, LAN, K.-H, TAMAOKI, T, OMATA, M, SHIRATORI, Y, TANAKA, T, OHASHI, M, OKUDAIRA, T, YOSHIDA, Y, WAKIMOTO, H, HAMADA, H, NAKABAYASHI, H
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA American Association for Cancer Research 01.02.1997
• Subjects: Adenoviridae - genetics; adenovirus; alpha-Fetoproteins - biosynthesis; Animals; Antineoplastic agents; Biological and medical sciences; Carcinoma, Hepatocellular - enzymology; Carcinoma, Hepatocellular - therapy; Chemotherapy; Cytosine Deaminase; Genetic Therapy; Humans; Liver Neoplasms - enzymology; Liver Neoplasms - therapy; Medical sciences; Mice; Mice, Inbred BALB C; Neoplasm Transplantation; Nucleoside Deaminases - genetics; Pharmacology. Drug treatments; Tumor Cells, Cultured; Antineoplastic agent; Adenoviridae; Cytosine deaminase; Hepatic disease; Hepatocellular carcinoma; Established cell line; Recombinant protein; Tumor cell; Fluorouracil; Human; Enzyme; Fluoropyrimidine derivatives; Rodentia; Malignant tumor; Gene expression; α-Fetoprotein; In vitro; Genetic transfer; Virus; In vivo; Vertebrata; Chemotherapy; Mammalia; Treatment; Mouse; Animal; Digestive diseases; Hydrolases; Gene therapy

The alpha-fetoprotein (AFP) gene is normally expressed in fetal liver and is transcriptionally silent in adult liver but overexpressed in human hepatocellular carcinoma (HCC). Here, we demonstrate that replication defective recombinant adenoviral vectors, containing the human AFP promoter/enhancer,...