Identification of Chromosomal Aberrations by Using Trypsin G-banding in Hepatocellular Carcinoma Patients (HCC) in Tamil Nadu, India
Hepatocellular carcinoma (HCC) (or liver cancer) is one of the most common human malignancies worldwide. Aetiologically, HCC is closely associated with chronic hepatitis B and C virus infection, cirrhosis and alcohol intake. The objective of the present study was to elucidate the chromosomal abberat...
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Published in | Tropical life sciences research Vol. 21; no. 1; pp. 31 - 46 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
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Malaysia
Penerbit Universiti Sains Malaysia
01.08.2010
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Abstract | Hepatocellular carcinoma (HCC) (or liver cancer) is one of the most common human malignancies worldwide. Aetiologically, HCC is closely associated with chronic hepatitis B and C virus infection, cirrhosis and alcohol intake. The objective of the present study was to elucidate the chromosomal abberations (CA) in HCC patients using the trypsin G-banding technique. This study may help in understanding the pattern of the disease and to assess whether these aberrations are associated with HCC susceptibility. The study examined 51 HCC cases and an equal number (n = 51) of age and gender matched cancer-free controls recruited from the hospitals in Tamil Nadu. The HCC cases were grouped depending upon their age into group I (≤ 45 years) and group II (≥ 46 years). The development of effective markers for the detection of HCC could have an impact on cancer mortality and may have significant public health implications worldwide. Subjects were recruited based on their alpha-fetoprotein (AFP) serum level, which is an effective marker for HCC. In the HCC cases, a higher number of chromatid aberrations [group I 13(25.5%) and group II 43(84.3%)] and CA [group I 10(19.6%) and group II 28(54.9%)] were observed. In contrast, controls showed a lower number of chromatid [group I 5(9.8%) and group II 12(23.5%)] and CA [group I 4(7.8%) and group II 9(17.6%)]. In conclusion, the results of this study contribute to the validation of CA as an intermediate end point in carcinogenesis. Because many people are unaware of this lethal disease, this study will raise awareness of this cancer. |
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AbstractList | Hepatocellular carcinoma (HCC) (or liver cancer) is one of the most common human malignancies worldwide. Aetiologically, HCC is closely associated with chronic hepatitis B and C virus infection, cirrhosis and alcohol intake. The objective of the present study was to elucidate the chromosomal abberations (CA) in HCC patients using the trypsin G-banding technique. This study may help in understanding the pattern of the disease and to assess whether these aberrations are associated with HCC susceptibility. The study examined 51 HCC cases and an equal number (n = 51) of age and gender matched cancer-free controls recruited from the hospitals in Tamil Nadu. The HCC cases were grouped depending upon their age into group I (≤ 45 years) and group II (≥ 46 years). The development of effective markers for the detection of HCC could have an impact on cancer mortality and may have significant public health implications worldwide. Subjects were recruited based on their alpha-fetoprotein (AFP) serum level, which is an effective marker for HCC. In the HCC cases, a higher number of chromatid aberrations [group I 13(25.5%) and group II 43(84.3%)] and CA [group I 10(19.6%) and group II 28(54.9%)] were observed. In contrast, controls showed a lower number of chromatid [group I 5(9.8%) and group II 12(23.5%)] and CA [group I 4(7.8%) and group II 9(17.6%)]. In conclusion, the results of this study contribute to the validation of CA as an intermediate end point in carcinogenesis. Because many people are unaware of this lethal disease, this study will raise awareness of this cancer. Hepatocellular carcinoma (HCC) (or liver cancer) is one of the most common human malignancies worldwide. Aetiologically, HCC is closely associated with chronic hepatitis B and C virus infection, cirrhosis and alcohol intake. The objective of the present study was to elucidate the chromosomal abberations (CA) in HCC patients using the trypsin G-banding technique. This study may help in understanding the pattern of the disease and to assess whether these aberrations are associated with HCC susceptibility. The study examined 51 HCC cases and an equal number ( n = 51) of age and gender matched cancer-free controls recruited from the hospitals in Tamil Nadu. The HCC cases were grouped depending upon their age into group I (≤ 45 years) and group II (≥ 46 years). The development of effective markers for the detection of HCC could have an impact on cancer mortality and may have significant public health implications worldwide. Subjects were recruited based on their alpha-fetoprotein (AFP) serum level, which is an effective marker for HCC. In the HCC cases, a higher number of chromatid aberrations [group I 13(25.5%) and group II 43(84.3%)] and CA [group I 10(19.6%) and group II 28(54.9%)] were observed. In contrast, controls showed a lower number of chromatid [group I 5(9.8%) and group II 12(23.5%)] and CA [group I 4(7.8%) and group II 9(17.6%)]. In conclusion, the results of this study contribute to the validation of CA as an intermediate end point in carcinogenesis. Because many people are unaware of this lethal disease, this study will raise awareness of this cancer. |
Author | Venkatakrishnan, Leela Krishnan Sasikala, Keshavarao Manikantan, Pappusamy Mohana Devi, Subramaniam Balachandar, Vellingiri Murugan, Palanivel Vel |
AuthorAffiliation | 3 Department of Gastroenterology, PSG Hospital, Coimbatore, Tamil Nadu, India 2 Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, South Korea 1 Human Genetics Laboratory, Department of Zoology, School of Life Science, Bharathiar University, Coimbatore-641046, Tamil Nadu, India |
AuthorAffiliation_xml | – name: 2 Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, South Korea – name: 3 Department of Gastroenterology, PSG Hospital, Coimbatore, Tamil Nadu, India – name: 1 Human Genetics Laboratory, Department of Zoology, School of Life Science, Bharathiar University, Coimbatore-641046, Tamil Nadu, India |
Author_xml | – sequence: 1 givenname: Subramaniam surname: Mohana Devi fullname: Mohana Devi, Subramaniam organization: Human Genetics Laboratory, Department of Zoology, School of Life Science, Bharathiar University, Coimbatore-641046, Tamil Nadu, India – sequence: 2 givenname: Vellingiri surname: Balachandar fullname: Balachandar, Vellingiri organization: Human Genetics Laboratory, Department of Zoology, School of Life Science, Bharathiar University, Coimbatore-641046, Tamil Nadu, India – sequence: 3 givenname: Palanivel Vel surname: Murugan fullname: Murugan, Palanivel Vel organization: Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, South Korea – sequence: 4 givenname: Keshavarao surname: Sasikala fullname: Sasikala, Keshavarao organization: Human Genetics Laboratory, Department of Zoology, School of Life Science, Bharathiar University, Coimbatore-641046, Tamil Nadu, India – sequence: 5 givenname: Pappusamy surname: Manikantan fullname: Manikantan, Pappusamy organization: Human Genetics Laboratory, Department of Zoology, School of Life Science, Bharathiar University, Coimbatore-641046, Tamil Nadu, India – sequence: 6 givenname: Leela Krishnan surname: Venkatakrishnan fullname: Venkatakrishnan, Leela Krishnan organization: Department of Gastroenterology, PSG Hospital, Coimbatore, Tamil Nadu, India |
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Title | Identification of Chromosomal Aberrations by Using Trypsin G-banding in Hepatocellular Carcinoma Patients (HCC) in Tamil Nadu, India |
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