VEGF isoforms and their expression after a single episode of hypoxia or repeated fluctuations between hyperoxia and hypoxia: relevance to clinical ROP

• Published in: Molecular vision Vol. 10; pp. 512 - 520
• Main Authors: McColm, Janet R, Geisen, Pete, Hartnett, M Elizabeth
• Format: Journal Article
• Language: English
• Published: United States 21.07.2004
• Subjects: Animals; Animals, Newborn; Enzyme-Linked Immunosorbent Assay; Eye Proteins; Female; Fluorescent Antibody Technique, Indirect; Gene Expression Regulation - physiology; Humans; Hyperoxia - metabolism; Hypoxia - metabolism; Infant, Newborn; Neovascularization, Pathologic - metabolism; Nerve Growth Factors; Oxygen - toxicity; Pregnancy; Protein Isoforms - genetics; Protein Isoforms - metabolism; Proteins - metabolism; Rats; Rats, Sprague-Dawley; Retinopathy of Prematurity - metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - metabolism; Serpins - metabolism; Up-Regulation; Vascular Endothelial Growth Factor A - genetics; Vascular Endothelial Growth Factor A - metabolism; Vitreous Body - blood supply

Fluctuations in oxygen are associated with the development of severe retinopathy of prematurity (ROP) in humans. However, the causal relationships between oxygen variability and severe ROP remain unknown. We investigated whether isoforms of vascular endothelial growth factor (VEGF) were differential...