An ultrastructural study of goblet cells in rat nasal mucosa as revealed by the quick-freezing method

• Published in: Journal of anatomy Vol. 188 ( Pt 3); no. Pt 3; pp. 651 - 659
• Main Authors: Shimomura, S, Hisamatsu, K, Fujii, Y, Ohno, S
• Format: Journal Article
• Language: English
• Published: England 01.06.1996
• Subjects: Acetylcholine - pharmacology; Animals; Cytoplasmic Granules - ultrastructure; Epithelium - drug effects; Epithelium - ultrastructure; Freeze Etching; Freeze Substitution; Male; Microscopy, Electron; Nasal Mucosa - cytology; Nasal Mucosa - drug effects; Nasal Mucosa - ultrastructure; Rats; Rats, Wistar; Substance P - pharmacology; Tissue Fixation

In order to clarify the natural ultrastructure of goblet cells in the rat nasal mucosa, they were examined by the quick-freezing and freeze-substitution (QF-FS) or deep-etching (QF-DE) methods for comparison with conventional fixation methods. Some nasal mucosal tissues were unstimulated; others wer...