Metabolomic Analysis of Toxoplasma gondii Tachyzoites

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 2071; p. 435
• Main Authors: King, Elizabeth F B, Cobbold, Simon A, Uboldi, Alessandro D, Tonkin, Christopher J, McConville, Malcolm J
• Format: Journal Article
• Language: English
• Published: United States 01.01.2020
• Subjects: Animals; Chromatography, Liquid; Fibroblasts - parasitology; Foreskin - cytology; Gas Chromatography-Mass Spectrometry; Humans; Male; Mass Spectrometry; Metabolomics; Software; Toxoplasma - metabolism; Toxoplasma - pathogenicity; Toxoplasmosis; Metabolomics; 13C-flux analysis; Toxoplasmosis; Intracellular pathogen; Mass spectrometry

This protocol describes the use of C-stable isotope labeling, combined with metabolite profiling, to investigate the metabolism of the tachyzoite stage of the protozoan parasite Toxoplasma gondii. T. gondii tachyzoites can infect any nucleated cell in their vertebrate (including human) hosts, and ut...