FISH on 3D preserved bovine and murine preimplantation embryos

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 659; p. 437
• Main Authors: Koehler, Daniela, Zakhartchenko, Valeri, Ketterl, Nina, Wolf, Eckhard, Cremer, Thomas, Brero, Alessandro
• Format: Journal Article
• Language: English
• Published: United States 2010
• Subjects: Animals; Blastocyst - cytology; Blastocyst - metabolism; Cattle; Imaging, Three-Dimensional; In Situ Hybridization, Fluorescence - methods; Mice; Microscopy, Fluorescence; Nucleic Acid Denaturation; Tissue Embedding; Tissue Fixation; Tissue Preservation - methods

Fluorescence in situ hybridization (FISH) is a commonly used technique for the visualization of whole chromosomes or subchromosomal regions, such as chromosome arms, bands, centromeres, or single gene loci. FISH is routinely performed on chromosome spreads, as well as on three-dimensionally preserve...