FRET-based biosensors for the detection and quantification of AI-2 class of quorum sensing compounds

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 692; p. 31
• Main Authors: Rajamani, Sathish, Sayre, Richard
• Format: Journal Article
• Language: English
• Published: United States 2011
• Subjects: Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - isolation & purification; Bacterial Proteins - metabolism; Biosensing Techniques - methods; Borates - analysis; Borates - metabolism; Culture Media; Electrophoresis, Polyacrylamide Gel; Fluorescence Resonance Energy Transfer - methods; Furans - analysis; Furans - metabolism; Homoserine - analogs & derivatives; Homoserine - metabolism; Lactones - metabolism; Models, Molecular; Protein Conformation; Quorum Sensing; Vibrio - cytology; Vibrio - metabolism

Intercellular small molecular weight signaling molecules modulate a variety of biological functions in bacteria. One of the more complex behaviors mediated by intercellular signaling molecules is the suite of activities regulated by quorum sensing molecules. These molecules mediate a variety of popu...