WST-8 analysis of cell viability during osteogenesis of human mesenchymal stem cells

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 740; p. 21
• Main Author: Stoddart, Martin J
• Format: Journal Article
• Language: English
• Published: United States 01.01.2011
• Subjects: Alkaline Phosphatase - metabolism; Cell Survival; Cells, Cultured; Cytological Techniques - methods; Humans; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - enzymology; Osteogenesis; Tetrazolium Salts - metabolism

WST-8 is one of the newer generation formazan-based dyes, which release the converted product into the medium in a soluble form. This allows for a non-destructive determination of viability enabling the cells to be subject to further investigations. This is a major advantage in cases where cell phen...