WST-8 analysis of cell viability during osteogenesis of human mesenchymal stem cells

WST-8 is one of the newer generation formazan-based dyes, which release the converted product into the medium in a soluble form. This allows for a non-destructive determination of viability enabling the cells to be subject to further investigations. This is a major advantage in cases where cell phen...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 740; p. 21
Main Author Stoddart, Martin J
Format Journal Article
LanguageEnglish
Published United States 01.01.2011
Subjects
Alkaline Phosphatase - metabolism
Cell Survival
Cells, Cultured
Cytological Techniques - methods
Humans
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - enzymology
Osteogenesis
Tetrazolium Salts - metabolism
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Summary:WST-8 is one of the newer generation formazan-based dyes, which release the converted product into the medium in a soluble form. This allows for a non-destructive determination of viability enabling the cells to be subject to further investigations. This is a major advantage in cases where cell phenotype is being investigated and data such as matrix synthesis is correlated to cell number. This chapter describes the use of WST-8 to normalise alkaline phosphatase activity.
ISSN:1940-6029
DOI:10.1007/978-1-61779-108-6_4