Bradykinin increases intracellular calcium levels in rat testis peritubular cells via the B2 receptor subtype

• Published in: British journal of pharmacology Vol. 138; no. 2; pp. 351 - 358
• Main Authors: Wennemuth, Gunther, Blöcher, Sonja, Schill, Wolf‐Bernhard, Aumüller, Gerhard, Monsees, Thomas K
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.01.2003; Nature Publishing
• Subjects: Animals; Biological and medical sciences; bradykinin; Bradykinin - pharmacology; Calcium - metabolism; Cell receptors; Cell structures and functions; Cells, Cultured; Fundamental and applied biological sciences. Psychology; intracellular calcium; Intracellular Fluid - drug effects; Intracellular Fluid - metabolism; Kinin B2 receptor; Male; male reproduction; Molecular and cellular biology; Neuropeptide receptors; peritubular cells; rat; Rats; Rats, Sprague-Dawley; Receptor, Bradykinin B2; Receptors, Bradykinin - agonists; Receptors, Bradykinin - metabolism; testis; Testis - cytology; Testis - drug effects; Testis - metabolism; Kinin B2 receptor; male reproduction; peritubular cells; Rat; Rodentia; Electrophysiology; bradykinin; Male; testis; Testicle; In vitro; B2 bradykinin receptor; Vertebrata; Reproduction; Mammalia; Calcium ion; Animal; intracellular calcium

RT–PCR and Western blots were used to detect bradykinin B2 receptors in testis and isolated peritubular cells of pre‐pubertal rats. RT–PCR demonstrated expression of a single transcript, whereas Western blots showed up to three specific bands that were in accordance with the described native, glycos...