Identification of XAGE-1 isoforms: predominant expression of XAGE-1b in testis and tumors
XAGE-1 is a cancer/testis (CT) antigen and has been shown to be immunogenic in lung cancer patients. Among 4 alternative splicing variants, XAGE-1a, b, c and d, XAGE-1b mRNA was dominantly expressed in cancer. In this study, we generated a XAGE-1b mAb, USO9-13. The B cell epitope recognized by the U...
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Published in | Cancer immunity Vol. 7; p. 5 |
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Main Authors | , , , , , , , , , , |
Format | Journal Article |
Language | English |
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United States
Academy of Cancer Immunology
05.03.2007
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Abstract | XAGE-1 is a cancer/testis (CT) antigen and has been shown to be immunogenic in lung cancer patients. Among 4 alternative splicing variants, XAGE-1a, b, c and d, XAGE-1b mRNA was dominantly expressed in cancer. In this study, we generated a XAGE-1b mAb, USO9-13. The B cell epitope recognized by the USO9-13 mAb was in the C-terminal region of the XAGE-1b protein and is also recognized by sera from patients with lung adenocarcinoma. Using USO9-13 and an anti-Flag mAb, we examined the translation products of the 4 transcripts. The XAGE-1a and b transcripts translated to the XAGE-1b protein. The XAGE-1c transcript possibly translated to 9- and 17-aa polypeptides. The XAGE-1d transcript translated to a protein consisting of 69 amino acids. Immunofluorescence analysis using USO9-13 mAb showed that the XAGE-1b protein is located in the nuclei of cells. Immunohistochemically, nuclear staining was heterogeneously observed in 25/47 lung adenocarcinomas, 1/12 hepatocellular carcinomas and 1/11 gastric cancers, but not in adjacent normal tissues. These findings suggested that XAGE-1b is a promising target molecule for a cancer vaccine against lung cancer. |
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AbstractList | XAGE-1 is a cancer/testis (CT) antigen and has been shown to be immunogenic in lung cancer patients. Among 4 alternative splicing variants, XAGE-1a, b, c and d, XAGE-1b mRNA was dominantly expressed in cancer. In this study, we generated a XAGE-1b mAb, USO9-13. The B cell epitope recognized by the USO9-13 mAb was in the C-terminal region of the XAGE-1b protein and is also recognized by sera from patients with lung adenocarcinoma. Using USO9-13 and an anti-Flag mAb, we examined the translation products of the 4 transcripts. The XAGE-1a and b transcripts translated to the XAGE-1b protein. The XAGE-1c transcript possibly translated to 9- and 17-aa polypeptides. The XAGE-1d transcript translated to a protein consisting of 69 amino acids. Immunofluorescence analysis using USO9-13 mAb showed that the XAGE-1b protein is located in the nuclei of cells. Immunohistochemically, nuclear staining was heterogeneously observed in 25/47 lung adenocarcinomas, 1/12 hepatocellular carcinomas and 1/11 gastric cancers, but not in adjacent normal tissues. These findings suggested that XAGE-1b is a promising target molecule for a cancer vaccine against lung cancer. XAGE-1 is a cancer/testis (CT) antigen and has been shown to be immunogenic in lung cancer patients. Among 4 alternative splicing variants, XAGE-1a , b , c and d , XAGE-1b mRNA was dominantly expressed in cancer. In this study, we generated a XAGE-1b mAb, USO9-13. The B cell epitope recognized by the USO9-13 mAb was in the C-terminal region of the XAGE-1b protein and is also recognized by sera from patients with lung adenocarcinoma. Using USO9-13 and an anti-Flag mAb, we examined the translation products of the 4 transcripts. The XAGE-1a and b transcripts translated to the XAGE-1b protein. The XAGE-1c transcript possibly translated to 9- and 17-aa polypeptides. The XAGE-1d transcript translated to a protein consisting of 69 amino acids. Immunofluorescence analysis using USO9-13 mAb showed that the XAGE-1b protein is located in the nuclei of cells. Immunohistochemically, nuclear staining was heterogeneously observed in 25/47 lung adenocarcinomas, 1/12 hepatocellular carcinomas and 1/11 gastric cancers, but not in adjacent normal tissues. These findings suggested that XAGE-1b is a promising target molecule for a cancer vaccine against lung cancer. |
Author | Shiratori, Yasushi Ohara, Nobuya Sato, Shuichiro Uenaka, Akiko Ishida, Toshiaki Nakayama, Eiichi Shimono, Michihide Noguchi, Yuji Nakagawa, Kazuhiko Koizumi, Fumihito Yoshino, Tadashi |
AuthorAffiliation | 2 Department of Gastroenterology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences Okayama Japan 1 Department of Immunology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences Okayama Japan 3 Department of Pathology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences Okayama Japan |
AuthorAffiliation_xml | – name: 2 Department of Gastroenterology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences Okayama Japan – name: 3 Department of Pathology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences Okayama Japan – name: 1 Department of Immunology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences Okayama Japan |
Author_xml | – sequence: 1 givenname: Shuichiro surname: Sato fullname: Sato, Shuichiro email: sato-s@md.okayama-u.ac.jp organization: Department of Immunology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Shikata-cho, Okayama, Japan. sato-s@md.okayama-u.ac.jp – sequence: 2 givenname: Yuji surname: Noguchi fullname: Noguchi, Yuji – sequence: 3 givenname: Nobuya surname: Ohara fullname: Ohara, Nobuya – sequence: 4 givenname: Akiko surname: Uenaka fullname: Uenaka, Akiko – sequence: 5 givenname: Michihide surname: Shimono fullname: Shimono, Michihide – sequence: 6 givenname: Kazuhiko surname: Nakagawa fullname: Nakagawa, Kazuhiko – sequence: 7 givenname: Fumihito surname: Koizumi fullname: Koizumi, Fumihito – sequence: 8 givenname: Toshiaki surname: Ishida fullname: Ishida, Toshiaki – sequence: 9 givenname: Tadashi surname: Yoshino fullname: Yoshino, Tadashi – sequence: 10 givenname: Yasushi surname: Shiratori fullname: Shiratori, Yasushi – sequence: 11 givenname: Eiichi surname: Nakayama fullname: Nakayama, Eiichi |
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Copyright | Copyright © 2007 by Shuichiro Sato |
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SubjectTerms | Adenocarcinoma - immunology Adenocarcinoma - metabolism Amino Acid Sequence Animals Antibodies, Monoclonal - biosynthesis Antigens, Neoplasm - immunology Antigens, Neoplasm - metabolism B-Lymphocytes - immunology Cell Nucleus - metabolism Cells, Cultured Female Humans Lung Neoplasms - immunology Lung Neoplasms - metabolism Male Mice Mice, Inbred BALB C Molecular Sequence Data Neoplasms - immunology Neoplasms - metabolism Protein Isoforms - metabolism Spermatocytes - metabolism Spermatogonia - metabolism Testis - immunology |
Title | Identification of XAGE-1 isoforms: predominant expression of XAGE-1b in testis and tumors |
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