Severe acute respiratory syndrome coronavirus ORF3a protein activates the NLRP3 inflammasome by promoting TRAF3-dependent ubiquitination of ASC

• Published in: The FASEB journal Vol. 33; no. 8; p. 8865
• Main Authors: Siu, Kam-Leung, Yuen, Kit-San, Castaño-Rodriguez, Carlos, Ye, Zi-Wei, Yeung, Man-Lung, Fung, Sin-Yee, Yuan, Shuofeng, Chan, Chi-Ping, Yuen, Kwok-Yung, Enjuanes, Luis, Jin, Dong-Yan
• Format: Journal Article
• Language: English
• Published: United States 01.08.2019
• Subjects: A549 Cells; Animals; CARD Signaling Adaptor Proteins - metabolism; Chlorocebus aethiops; HEK293 Cells; Humans; Inflammasomes - metabolism; NLR Family, Pyrin Domain-Containing 3 Protein - metabolism; SARS Virus - metabolism; TNF Receptor-Associated Factor 3 - metabolism; Ubiquitination; Vero Cells; Viral Structural Proteins - metabolism; SARS coronavirus

Severe acute respiratory syndrome coronavirus (SARS-CoV) is capable of inducing a storm of proinflammatory cytokines. In this study, we show that the SARS-CoV open reading frame 3a (ORF3a) accessory protein activates the NLRP3 inflammasome by promoting TNF receptor-associated factor 3 (TRAF3)-mediat...