Generating lineage-resolved, complete metagenome-assembled genomes from complex microbial communities

• Published in: Nature biotechnology Vol. 40; no. 5; pp. 711 - 719
• Main Authors: Bickhart, Derek M., Kolmogorov, Mikhail, Tseng, Elizabeth, Portik, Daniel M., Korobeynikov, Anton, Tolstoganov, Ivan, Uritskiy, Gherman, Liachko, Ivan, Sullivan, Shawn T., Shin, Sung Bong, Zorea, Alvah, Andreu, Victòria Pascal, Panke-Buisse, Kevin, Medema, Marnix H., Mizrahi, Itzhak, Pevzner, Pavel A., Smith, Timothy P. L.
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.05.2022; Nature Publishing Group
• Subjects: 631/114/2785; 631/208/728; 631/326/325/2482; Agriculture; Bioinformatics; Biomedical and Life Sciences; Biomedical Engineering/Biotechnology; Biomedicine; biosynthesis; Biotechnology; data collection; Gene clusters; Genomes; Haplotypes; Life Sciences; Metagenomics; Microbial activity; Microbiomes; Microorganisms; sheep

Microbial communities might include distinct lineages of closely related organisms that complicate metagenomic assembly and prevent the generation of complete metagenome-assembled genomes (MAGs). Here we show that deep sequencing using long (HiFi) reads combined with Hi-C binning can address this ch...