How leachates from wasted cigarette butts influence aquatic life? A case study on freshwater mussel Anodontites trapesiali

• Published in: The Science of the total environment Vol. 689; pp. 381 - 389
• Main Authors: Montalvão, Mateus Flores, Chagas, Thales Quintão, da Silva Alvarez, Tenilce Gabriela, Mesak, Carlos, da Costa Araújo, Amanda Pereira, Gomes, Alex Rodrigues, de Andrade Vieira, Julya Emmanuela, Malafaia, Guilherme
• Format: Journal Article
• Language: English
• Published: Netherlands 01.11.2019
• Subjects: adults; Animals; Anodontites; bioaccumulation; Bivalvia - drug effects; case studies; chemical composition; chromium; cigarettes; cytoplasm; Dose-Response Relationship, Drug; Environmental Monitoring; freshwater mussels; granulocytes; Hemocytes - drug effects; hemolymph; human health; immunotoxicity; leachates; lead; manganese; morphometry; mutagenicity; mutagens; nickel; phagocytes; pollutants; sodium; Tobacco Products; Water Pollutants, Chemical - toxicity; zinc; Hemolymph; Aquatic pollution; Mollusks; Mutagenicity; Cigarette buds
• DOI: 10.1016/j.scitotenv.2019.06.385
• ISSN: 1879-1026; 0048-9697

There are several reports on the damage smoking causes to human health available in the literature, but little is known about the environmental and biological consequences from inappropriate cigarette butt (CB) disposal in urban and natural environments. The immunotoxic, morphotoxic and mutagenic potential of leachates from cigarette butts (LCB) diluted at environmentally relevant rates (LCB1x: 1.375%; LCB10x: 13.75%) was evaluated in adult representatives of the bivalve species Anodontites trapesialis, which was adopted as model organism. Type II hyalinocytes and granulocytes (phagocytic cells) frequency increased in the hemolymph of subjects exposed to the pollutant for 14 days. Based on this outcome, LCB chemical constituents did not induce immunotoxic effects. The treatments also did not seem to have any impact on the subjects' hemocitary morphometry parameters: diameter, area, perimeter, circularity and nucleus - cytoplasm ratio. However, subjects in groups LCB1x and LCB10x recorded a larger number of hyalinocytes with some nuclear abnormality such as micronucleus, blebbed nucleus, asymmetric constriction nucleus, and nuclear multilobulation and binucleation. The association between these abnormalities and the treatments was confirmed by the Cr, Ni, Pb, Zn, Mn and Na bioaccumulation in tissue samples of the bivalve models exposed to LCB. To the best of our knowledge, this is the first report on LCB mutagenicity in representatives of a freshwater bivalve group. Given the chemical complexity of the addressed pollutants, it is imperative to develop further investigations about the topic.