Purification and biochemical characterization of a mycelial alkaline phosphatase without DNAase activity produced by Aspergillus caespitosus

• Published in: Folia microbiologica Vol. 52; no. 3; pp. 231 - 236
• Main Authors: Guimarães, L H S, Júnior, A B, Jorge, J A, Terenzi, H F, Polizeli, M L T M
• Format: Journal Article
• Language: English
• Published: United States 2007
• Subjects: Alkaline Phosphatase - chemistry; Alkaline Phosphatase - isolation & purification; Alkaline Phosphatase - metabolism; Aspergillus; Aspergillus - enzymology; Deoxyribonucleases - metabolism; Kinetics; Mycelium - enzymology; Substrate Specificity

Biochemical properties of a termostable alkaline phosphatase obtained from the mycelium extract of A. caespitosus were described. The enzyme was purified 42-fold with 32% recovery by DEAE-cellulose and concanavalin A-Sepharose chromatography. The molar mass estimated by Sephacryl S-200 or by 7% SDS-...