Effect of protein kinase inhibitors on protein phosphorylation and germination of aerial spores from Streptomyces coelicolor

• Published in: Folia microbiologica Vol. 52; no. 3; pp. 215 - 222
• Main Authors: Palecková, P, Kontrová, F, Kofronová, O, Bobek, J, Benada, O, Mikulík, K
• Format: Journal Article
• Language: English
• Published: United States 2007
• Subjects: Bacterial Proteins - metabolism; Phosphorylation - drug effects; Protein Kinase Inhibitors - pharmacology; Protein Kinases - drug effects; Protein Kinases - metabolism; Spores, Bacterial - drug effects; Spores, Bacterial - metabolism; Streptomyces coelicolor; Streptomyces coelicolor - drug effects; Streptomyces coelicolor - enzymology; Streptomyces coelicolor - growth & development

In vitro phosphorylation reaction using extracts prepared from cells in the exponential phase of growth and aerial spores of Streptomyces coelicolor displayed the presence of multiply phosphorylated proteins. Effect of protein kinase inhibitors (PKIs) (geldanamycin, wortmannin, apigenin, genistein,...