Metabolism of PER.C6™ cells cultivated under fed-batch conditions at low glucose and glutamine levels

• Published in: Biotechnology and bioengineering Vol. 94; no. 1; pp. 139 - 150
• Main Authors: MARANGA, Luis, GOOCHEE, Charles F
• Format: Journal Article
• Language: English
• Published: New York, NY Wiley 05.05.2006
• Subjects: Adenoviridae - genetics; Amino Acids - metabolism; Ammonia - metabolism; Biological and medical sciences; Bioreactors; Biotechnology; Cell Count; Cell Culture Techniques; Cell Line, Transformed; Cell Survival; Cell Transformation, Viral; Culture Media - chemistry; Culture Media, Serum-Free; Energy Metabolism; Fundamental and applied biological sciences. Psychology; Glucose - metabolism; Glutamine - metabolism; Humans; Lactic Acid - metabolism; Oxygen - metabolism; Retina - cytology; Retina - embryology; Retina - growth & development; Retina - metabolism; Retina - virology; Time Factors; Human; Cell culture; Cell line; fed-batch; metabolic shift; PER.C6# cells; Glucose; Metabolism; Semicontinuous; Glutamine

This is the first study to examine PER.C6 cell glucose/energy and glutamine metabolism with fed-batch cultures at controlled low glutamine, low glucose, and simultaneous low glucose and low glutamine levels. PER.C6(TM) cell metabolism was investigated in serum-free suspension bioreactors at two-lite...