Structural and biochemical characterisation of Co2+-binding sites on serum albumins and their interplay with fatty acids

• Published in: Chemical science (Cambridge) Vol. 14; no. 23; pp. 6244 - 6258
• Main Authors: Wu, Dongmei, Gucwa, Michal, Czub, Mateusz P, Cooper, David R, Shabalin, Ivan G, Fritzen, Remi, Arya, Swati, Schwarz-Linek, Ulrich, Blindauer, Claudia A, Minor, Wladek, Stewart, Alan J
• Format: Journal Article
• Language: English
• Published: Cambridge Royal Society of Chemistry 14.06.2023; The Royal Society of Chemistry
• Subjects: Affinity; Binding sites; Chemistry; Cobalt; Crystal structure; Crystallography; Esterification; Fatty acids; Ischemia; Physiological effects; Serum albumin; Structural analysis; Titration calorimetry; Toxicity

Serum albumin–Co2+ interactions are of clinical importance. They play a role in mediating the physiological effects associated with cobalt toxicity and are central to the albumin cobalt binding (ACB) assay for diagnosis of myocardial ischemia. To further understand these processes, a deeper understa...