Thermotoga maritima 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase: the ancestral eubacterial DAHP synthase?

• Published in: The Journal of biological chemistry Vol. 278; no. 30; pp. 27525 - 27531
• Main Authors: Wu, Jing, Howe, David L, Woodard, Ronald W
• Format: Journal Article
• Language: English
• Published: United States 25.07.2003
• Subjects: 3-Deoxy-7-Phosphoheptulonate Synthase; Aldehyde-Lyases - chemistry; Aldehyde-Lyases - genetics; Aldehyde-Lyases - metabolism; Amino Acid Sequence; Cloning, Molecular; Edetic Acid - pharmacology; Electrophoresis, Polyacrylamide Gel; Escherichia coli - metabolism; Hydrogen-Ion Concentration; Ions; Kinetics; Molecular Sequence Data; Plasmids - metabolism; Protein Denaturation; Sequence Homology, Amino Acid; Temperature; Thermotoga maritima - enzymology; Time Factors; Zinc - chemistry

The gene encoding the 3-deoxy-d-arabino-heptulosonate 7-phosphate (DAHP) synthase from the thermophilic microorganism Thermotoga maritima was cloned, and the enzyme was overexpressed in Escherichia coli. The purified DAHP synthase displays a homotetrameric structure and exhibits maximal activity at...