Excision of 3' termini by the Trex1 and TREX2 3'-->5' exonucleases. Characterization of the recombinant proteins

• Published in: The Journal of biological chemistry Vol. 276; no. 20; pp. 17022 - 17029
• Main Authors: Mazur, D J, Perrino, F W
• Format: Journal Article
• Language: English
• Published: United States 18.05.2001
• Subjects: Animals; Chlorides - pharmacology; Cloning, Molecular; DNA - metabolism; DNA, Single-Stranded - metabolism; Endodeoxyribonucleases - genetics; Endodeoxyribonucleases - metabolism; Escherichia coli; Exodeoxyribonuclease V; Exodeoxyribonucleases - genetics; Exodeoxyribonucleases - metabolism; Humans; Hydrogen-Ion Concentration; Kinetics; Magnesium Chloride - pharmacology; Mammals; Manganese Compounds - pharmacology; Mice; Peptide Fragments - metabolism; Phosphoproteins; Recombinant Proteins - chemistry; Recombinant Proteins - metabolism; Substrate Specificity; TREX1 protein; TREX2 protein

The excision of nucleotides from DNA 3' termini is an important step in DNA replication, repair, and recombination pathways to generate correctly base paired termini for subsequent processing. The mammalian TREX1 and TREX2 proteins contain potent 3'-->5' exonucleases capable of fun...