Molecular basis defining human Chlamydia trachomatis tissue tropism. A possible role for tryptophan synthase

• Published in: The Journal of biological chemistry Vol. 277; no. 30; pp. 26893 - 26903
• Main Authors: Fehlner-Gardiner, Christine, Roshick, Christine, Carlson, John H, Hughes, Scott, Belland, Robert J, Caldwell, Harlan D, McClarty, Grant
• Format: Journal Article
• Language: English
• Published: United States 26.07.2002
• Subjects: Amino Acid Sequence; Binding Sites; Blotting, Western; Cells, Cultured; Chlamydia trachomatis - enzymology; Chlamydia trachomatis - physiology; Cloning, Molecular; Escherichia coli - metabolism; Genetic Complementation Test; Glycerophosphates - metabolism; HeLa Cells; Humans; Models, Biological; Molecular Sequence Data; Mutation; Plasmids - metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - metabolism; Salmonella - metabolism; Sequence Homology, Amino Acid; Transcription, Genetic; Tryptophan - metabolism; Tryptophan Synthase - physiology

Here we report the cloning and sequencing of a region of the chlamydiae chromosome termed the "plasticity zone" from all the human serovars of C. trachomatis containing the tryptophan biosynthesis genes. Our results show that this region contains orthologues of the tryptophan repressor as...