Mutagenicity of methionine or its metabolic products in RSV-transformed Chinese hamster fibroblasts

Methionine has been successfully used to control tumor progression in vivo and to induce reversions of transformed cells in vitro. In the present study, we measured mutations at the HGPRT locus of RSV-transformed cells serially propagated in methionine-supplemented medium and assayed at each passage...

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Bibliographic Details
Published inAnticancer research Vol. 7; no. 6; p. 1245
Main Authors Hill, M, Hillova, J, Brada, Z, Mariage-Samson, R
Format Journal Article
LanguageEnglish
Published Greece 01.11.1987
Subjects
Animals
Avian Sarcoma Viruses
Cell Line, Transformed
Chromosome Mapping
Cricetinae
Cricetulus
DNA Repair
Fibroblasts - drug effects
Fibroblasts - metabolism
Hypoxanthine Phosphoribosyltransferase - genetics
Methionine - metabolism
Methionine - toxicity
Mutagenicity Tests
Mutagens
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Summary:Methionine has been successfully used to control tumor progression in vivo and to induce reversions of transformed cells in vitro. In the present study, we measured mutations at the HGPRT locus of RSV-transformed cells serially propagated in methionine-supplemented medium and assayed at each passage for thioguanine resistance. The frequency of spontaneous mutants at this locus was 7.2 X 10(-5); this value gradually increased during the methionine treatment to as much as 9.2 X 10(-4), and returned to initial values when the methionine treatment was withdrawn. It is proposed that the mutants were induced by the methionine derivative, S-adenosylmethionine, and the resulting mutant frequency determined by equilibrium between mutagenic action of this metabolite and DNA repair.
ISSN:0250-7005