Arginine aminopeptidase activity of phytopathogenic spiroplasmas

The arginine aminopeptidase activity of arginine-utilizing phytopathogenic spiroplasmas was investigated with arginine beta-naphthylamide substrate using the fluorometric method. Hydrolysis of this substrate was demonstrated with broth cultures, washed concentrated whole cells, and cell-free extract...

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Published inIsrael journal of medical sciences Vol. 20; no. 10; p. 1022
Main Authors Stevens, C, Cody, R M, Gudauskas, R T, Patterson, A
Format Journal Article
LanguageEnglish
Published Israel 01.10.1984
Subjects
Aminopeptidases - biosynthesis
Aminopeptidases - metabolism
Arginine - metabolism
Enzyme Repression
Plants - microbiology
Spiroplasma - enzymology
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Abstract The arginine aminopeptidase activity of arginine-utilizing phytopathogenic spiroplasmas was investigated with arginine beta-naphthylamide substrate using the fluorometric method. Hydrolysis of this substrate was demonstrated with broth cultures, washed concentrated whole cells, and cell-free extracts of corn stunt spiroplasma (CSS) and Spiroplasma citri. Growing CSS and S. citri in the presence of 47 mM arginine resulted in a reduction in aminopeptidase activity, indicating that synthesis of the enzyme might be subject to control by catabolic repression. Results of these experiments suggest a possible biochemical basis for pathogenicity of phytopathogenic spiroplasmas in vivo.
AbstractList The arginine aminopeptidase activity of arginine-utilizing phytopathogenic spiroplasmas was investigated with arginine beta-naphthylamide substrate using the fluorometric method. Hydrolysis of this substrate was demonstrated with broth cultures, washed concentrated whole cells, and cell-free extracts of corn stunt spiroplasma (CSS) and Spiroplasma citri. Growing CSS and S. citri in the presence of 47 mM arginine resulted in a reduction in aminopeptidase activity, indicating that synthesis of the enzyme might be subject to control by catabolic repression. Results of these experiments suggest a possible biochemical basis for pathogenicity of phytopathogenic spiroplasmas in vivo.
Author Cody, R M
Stevens, C
Gudauskas, R T
Patterson, A
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SubjectTerms Aminopeptidases - biosynthesis
Aminopeptidases - metabolism
Arginine - metabolism
Enzyme Repression
Plants - microbiology
Spiroplasma - enzymology
Title Arginine aminopeptidase activity of phytopathogenic spiroplasmas
URI https://www.ncbi.nlm.nih.gov/pubmed/6511313
Volume 20
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