A simplified procedure for purification of bovine growth hormone on Sephacryl S-200 superfine

The isolation and purification of bovine growth hormone from pituitary glands by a shorter and efficient sequence of stages which utilizes the Reichert approach (Reichert, 1975) is described. The major innovations are: 1) precipitation in 2 M ammonium sulfate at pH 7.9 instead of pH 7.0 to obtain th...

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Bibliographic Details
Published inThe Italian journal of biochemistry Vol. 30; no. 2; p. 117
Main Authors Secchi, C, Berrini, A, Signorelli, V, Biondi, P A
Format Journal Article
LanguageEnglish
Published Italy 01.01.1981
Subjects
Acrylic Resins
Animals
Cattle
Chromatography, Gel - methods
Electrophoresis, Polyacrylamide Gel
Growth Hormone - isolation & purification
Isoelectric Focusing
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Summary:The isolation and purification of bovine growth hormone from pituitary glands by a shorter and efficient sequence of stages which utilizes the Reichert approach (Reichert, 1975) is described. The major innovations are: 1) precipitation in 2 M ammonium sulfate at pH 7.9 instead of pH 7.0 to obtain the extract designated as C-2 by Reichert and 2) early gel-chromatography on Sephacryl S-200 of the C-2 extract. The bGH obtained has higher specific activity than the standard reference hormone NIH-GH-B16. Electrophoretic and size homogeneity is investigated by isoelectric focusing and sodium dodecyl sulfate electrophoresis.
ISSN:0021-2938