A new member of the Eph family of receptors that lacks protein tyrosine kinase activity

Using a PCR-based screen to identify tyrosine kinases involved in T cell development, we have cloned a new member of the Eph-family of receptor tyrosine kinases (Mep, for murine eph-family protein). At the amino acid level Mep is 60% identical to the chicken embryonic kinase Cek9. Sequence analysis...

Full description

Saved in:
Bibliographic Details
Published inOncogene Vol. 13; no. 4; p. 777
Main Authors Gurniak, C B, Berg, L J
Format Journal Article
LanguageEnglish
Published England 15.08.1996
Subjects
Online AccessGet more information

Cover

Loading…
Abstract Using a PCR-based screen to identify tyrosine kinases involved in T cell development, we have cloned a new member of the Eph-family of receptor tyrosine kinases (Mep, for murine eph-family protein). At the amino acid level Mep is 60% identical to the chicken embryonic kinase Cek9. Sequence analysis indicates that the predicted extracellular portion of Mep bears an Ig-like domain, a cysteine-rich region, and sequences homologous to fibronectin type III. The transmembrane region of Mep is followed by a kinase domain. Surprisingly, this kinase domain carries amino acid substitutions in the highly conserved consensus motifs found in all protein tyrosine kinases and known to be crucial for kinase activity. We demonstrate that a bacterial fusion protein of the Mep kinase domain does not have protein tyrosine kinase activity. Analysis of Mep mRNA levels in a variety of mouse tissues shows that Mep is highly expressed in thymus and brain. We have also isolated two additional Mep cDNA clones from thymocytes which are predicted to encode secreted forms of the Mep extracellular domain; mRNAs encoding these secreted isoforms are also expressed in mouse brain.
AbstractList Using a PCR-based screen to identify tyrosine kinases involved in T cell development, we have cloned a new member of the Eph-family of receptor tyrosine kinases (Mep, for murine eph-family protein). At the amino acid level Mep is 60% identical to the chicken embryonic kinase Cek9. Sequence analysis indicates that the predicted extracellular portion of Mep bears an Ig-like domain, a cysteine-rich region, and sequences homologous to fibronectin type III. The transmembrane region of Mep is followed by a kinase domain. Surprisingly, this kinase domain carries amino acid substitutions in the highly conserved consensus motifs found in all protein tyrosine kinases and known to be crucial for kinase activity. We demonstrate that a bacterial fusion protein of the Mep kinase domain does not have protein tyrosine kinase activity. Analysis of Mep mRNA levels in a variety of mouse tissues shows that Mep is highly expressed in thymus and brain. We have also isolated two additional Mep cDNA clones from thymocytes which are predicted to encode secreted forms of the Mep extracellular domain; mRNAs encoding these secreted isoforms are also expressed in mouse brain.
Author Gurniak, C B
Berg, L J
Author_xml – sequence: 1
  givenname: C B
  surname: Gurniak
  fullname: Gurniak, C B
  organization: Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
– sequence: 2
  givenname: L J
  surname: Berg
  fullname: Berg, L J
BackLink https://www.ncbi.nlm.nih.gov/pubmed/8761299$$D View this record in MEDLINE/PubMed
BookMark eNotj8tqwzAUBbVISZO0n1DQDxiuJVuSlyGkDwhk09Jl0OOaqLFlIakt_vu2NKsDMzBw1mQRpoALsoKuhapjnN2Sdc4fACA7YEuyVFLUrOtW5H1LA37TEUeDiU49LWek-3imvR79MP-RhBZjmVL-dbrQQdtLpjFNBX2gZU5T9gHpxQedkWpb_Jcv8x256fWQ8f66G_L2uH_dPVeH49PLbnuoIuOyVByssqyRzgimOUdQTgnHVSONkAi94V1TN22LTrYNOAQEw1mrmBYga2HZhjz8d-OnGdGdYvKjTvPpepD9APTCTSk
ContentType Journal Article
DBID CGR
CUY
CVF
ECM
EIF
NPM
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
DatabaseTitleList MEDLINE
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod no_fulltext_linktorsrc
Discipline Medicine
Chemistry
Biology
ExternalDocumentID 8761299
Genre Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S
Journal Article
GroupedDBID ---
.55
.GJ
123
36B
3O-
53G
5RE
ABAWZ
ABEFU
ABJNI
ABLJU
ACGFO
ACGFS
ACMJI
ACPRK
ADFRT
AENEX
AFFNX
AHMBA
ALIPV
ALMA_UNASSIGNED_HOLDINGS
CGR
CS3
CUY
CVF
DU5
ECM
EIF
ESX
F5P
IAO
IHR
L7B
N9A
NPM
NXXTH
P2P
RNT
TSG
UDS
WH7
X7M
ZXP
ID FETCH-LOGICAL-p237t-30c8c247db62a33e08d86d3847b67e0fb3941455ed7540de0e0b32582a60716c2
ISSN 0950-9232
IngestDate Tue Oct 15 23:16:05 EDT 2024
IsPeerReviewed true
IsScholarly true
Issue 4
Language English
LinkModel OpenURL
MergedId FETCHMERGED-LOGICAL-p237t-30c8c247db62a33e08d86d3847b67e0fb3941455ed7540de0e0b32582a60716c2
PMID 8761299
ParticipantIDs pubmed_primary_8761299
PublicationCentury 1900
PublicationDate 1996-08-15
PublicationDateYYYYMMDD 1996-08-15
PublicationDate_xml – month: 08
  year: 1996
  text: 1996-08-15
  day: 15
PublicationDecade 1990
PublicationPlace England
PublicationPlace_xml – name: England
PublicationTitle Oncogene
PublicationTitleAlternate Oncogene
PublicationYear 1996
SSID ssj0007902
Score 1.8538699
Snippet Using a PCR-based screen to identify tyrosine kinases involved in T cell development, we have cloned a new member of the Eph-family of receptor tyrosine...
SourceID pubmed
SourceType Index Database
StartPage 777
SubjectTerms Alternative Splicing
Amino Acid Sequence
Animals
Base Sequence
Brain - metabolism
Chickens
DNA, Complementary
Membrane Proteins - genetics
Membrane Proteins - metabolism
Mice
Molecular Sequence Data
Protein-Tyrosine Kinases - metabolism
Receptor, EphB6
RNA, Messenger - genetics
RNA, Messenger - metabolism
Sequence Homology, Amino Acid
Thymus Gland - metabolism
Title A new member of the Eph family of receptors that lacks protein tyrosine kinase activity
URI https://www.ncbi.nlm.nih.gov/pubmed/8761299
Volume 13
hasFullText
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnZ1JS8NAFMcHq7hcRKvizhy8lciQSTPJsUq1iMvFYm-S2ahI04DxoJ_eN0vSWBSXSwgzbVPml8y8eXnv_RE6ibUwWm9hoGQ3DiIlRZBxqQNCRFco2BNpbQNkb-PBMLoadUczbU6bXVLyU_H-ZV7Jf6hCG3A1WbJ_IFv_KDTAOfCFIxCG468Y94wgeGeijKhH9bK_X4wrrwW0wHymCiuoU46zsmO8dS8dW5vBxDe-wRJprMznpxwWM1tXw0hJNA3Wu1xM4fo1_stXk8dlp9CGYvOZDxK79m-ZpM-ri41f1CVS1v5AEoC193lqpI1bIGrMc8xprzTGuJjYQYbJFSyI9MfOuSLXvqeFWiwxAhy3xufi11PmIkfrP7iGlv3H57YC1iS430Dr3pbHPQdmEy2ovI2WnbrnWxutnldiem20cuNjGLbQQw8DNeyo4anGQA0DNeyomZaaGjbUsKWGPTVcUcOOGq6obaPhRf_-fBB4eYugCCkrA0pEIsKISR6HGaWKJDKJJQVzgcdMEc1pGpky8koyMKulIopwGnaTMDM1AWMR7qDFfJqrXYQTLjmF78B2NIy0ZBnVNEoVy1IJO4KI7KEdN1CPhath8uhHcP-7jgO0NrtLDtGShkdGHYH9VfJji-cDcWoyNA
link.rule.ids 780
linkProvider National Library of Medicine
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=A+new+member+of+the+Eph+family+of+receptors+that+lacks+protein+tyrosine+kinase+activity&rft.jtitle=Oncogene&rft.au=Gurniak%2C+C+B&rft.au=Berg%2C+L+J&rft.date=1996-08-15&rft.issn=0950-9232&rft.volume=13&rft.issue=4&rft.spage=777&rft_id=info%3Apmid%2F8761299&rft_id=info%3Apmid%2F8761299&rft.externalDocID=8761299
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0950-9232&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0950-9232&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0950-9232&client=summon