Identification of a 43-kDa protein in human liver cytosol that binds to the 3'-untranslated region of CYP2A6 mRNA

• Published in: Biochemical pharmacology Vol. 62; no. 6; pp. 669 - 678
• Main Authors: GILMORE, James, ROTONDO, Francesca, PELLETIER, Anne-Marie, LAMARRE, Jonathan, ALAOUI-JAMALI, Moulay, KIRBY, Gordon M
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Science 15.09.2001
• Subjects: 3' Untranslated Regions - metabolism; Analytical, structural and metabolic biochemistry; Aryl Hydrocarbon Hydroxylases; Base Sequence; Binding and carrier proteins; Biological and medical sciences; Cytochrome P-450 CYP2A6; Cytochrome P-450 Enzyme System - genetics; Cytochrome P-450 Enzyme System - metabolism; Cytochrome P450 Family 2; Cytosol - metabolism; Fundamental and applied biological sciences. Psychology; Humans; In Vitro Techniques; Microsomes, Liver - metabolism; Mixed Function Oxygenases - genetics; Mixed Function Oxygenases - metabolism; Molecular Sequence Data; Molecular Weight; Proteins; RNA, Messenger - analysis; RNA, Messenger - metabolism; RNA-Binding Proteins - isolation & purification; RNA-Binding Proteins - metabolism; Human; RNA; Transcription; Digestive system; Cytochrome P450; Liver; Molecular interaction; Gene expression; Microsome; Cytosol; In vitro; Binding protein; Biological fixation; Aminoacid sequence

Hepatic expression of cytochrome P450 2A6 (CYP2A6) varies widely in humans and is induced during hepatitis; however, the mechanism regulating CYP2A6 has not been established. The murine orthologue Cyp2a5 is regulated post-transcriptionally by mRNA stabilization. A 43-kDa protein that binds to the 3&...