The effect of crotoxin on the release of acetylcholine and lactate dehydrogenase from rat brain cortical slices
1. We have studied the effects of crotoxin, the neurotoxin of the South American rattlesnake Crotalus durissus terrificus, on the release of acetylcholine and lactate dehydrogenase from rat brain cortical slices. 2. Crotoxin enhances the release of [3H]-acetylcholine from cortical slices (control va...
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Published in | Brazilian journal of medical and biological research Vol. 25; no. 7; pp. 707 - 711 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Ribeirão Preto
Associação Brasileira de Divulgação Científica
1992
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Subjects | |
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Abstract | 1. We have studied the effects of crotoxin, the neurotoxin of the South American rattlesnake Crotalus durissus terrificus, on the release of acetylcholine and lactate dehydrogenase from rat brain cortical slices. 2. Crotoxin enhances the release of [3H]-acetylcholine from cortical slices (control values 92.8 +/- 5.9 and 150.3 +/- 11.7 DPM/mg and crotoxin values 199.1 +/- 7.0 and 336.0 +/- 26.0 DPM/mg, at 60 and 120 min incubation, respectively) in parallel with the release of lactate dehydrogenase (control values 50.4 +/- 16.8 and 80.3 +/- 19.5 U/mg and crotoxin values 162.5 +/- 39.1 and 355.7 +/- 38.2 U/mg, at 60 and 120 min incubation, respectively). Both effects are markedly reduced when substituting Sr2+ for Ca2+ in the incubation medium. 3. It is concluded that the phospholipase activity of crotoxin is responsible for the observed effects. |
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AbstractList | 1. We have studied the effects of crotoxin, the neurotoxin of the South American rattlesnake Crotalus durissus terrificus, on the release of acetylcholine and lactate dehydrogenase from rat brain cortical slices. 2. Crotoxin enhances the release of [3H]-acetylcholine from cortical slices (control values 92.8 +/- 5.9 and 150.3 +/- 11.7 DPM/mg and crotoxin values 199.1 +/- 7.0 and 336.0 +/- 26.0 DPM/mg, at 60 and 120 min incubation, respectively) in parallel with the release of lactate dehydrogenase (control values 50.4 +/- 16.8 and 80.3 +/- 19.5 U/mg and crotoxin values 162.5 +/- 39.1 and 355.7 +/- 38.2 U/mg, at 60 and 120 min incubation, respectively). Both effects are markedly reduced when substituting Sr2+ for Ca2+ in the incubation medium. 3. It is concluded that the phospholipase activity of crotoxin is responsible for the observed effects. |
Author | BEIRAO, P. S. L ARAUJO, D. A. M GOMEZ, M. V |
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Keywords | Rat Enzyme Toxicity Rodentia Lactate dehydrogenase Ophidia Vertebrata Mammalia Neuromediator Venom Reptilia Acetylcholine Brain (vertebrata) |
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Snippet | 1. We have studied the effects of crotoxin, the neurotoxin of the South American rattlesnake Crotalus durissus terrificus, on the release of acetylcholine and... |
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SubjectTerms | Acetylcholine - analysis Acetylcholine - secretion Animals Autoradiography Biological and medical sciences Brain Chemistry - drug effects Calcium - pharmacology Cerebral Cortex - chemistry Cerebral Cortex - drug effects Cerebral Cortex - secretion Crotoxin - pharmacology In Vitro Techniques L-Lactate Dehydrogenase - analysis L-Lactate Dehydrogenase - drug effects L-Lactate Dehydrogenase - secretion Medical sciences Rats Strontium - pharmacology Time Factors Toxicology Tritium |
Title | The effect of crotoxin on the release of acetylcholine and lactate dehydrogenase from rat brain cortical slices |
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