Human alveolar macrophage subpopulations isolated on discontinuous albumin gradients. Cytological data in normals and sarcoid patients

• Published in: European journal of respiratory diseases Vol. 68; no. 3; p. 177
• Main Authors: Sandron, D, Reynolds, H Y, Laval, A M, Venet, A, Israel-Biet, D, Chretien, J
• Format: Journal Article
• Language: English
• Published: Denmark 01.03.1986
• Subjects: Adult; Bronchi - cytology; Bronchi - pathology; Cell Separation; Centrifugation, Density Gradient; Female; Histocytochemistry; Humans; Isoenzymes - metabolism; Macrophages - classification; Macrophages - enzymology; Male; Peroxidase; Peroxidases - metabolism; Pulmonary Alveoli - cytology; Pulmonary Alveoli - enzymology; Pulmonary Alveoli - pathology; Sarcoidosis - pathology; Serum Albumin, Bovine; Therapeutic Irrigation

Cells recovered by bronchoalveolar lavage (BAL) from ten subjects (five normals and five sarcoid patients) were centrifuged on discontinuous albumin gradients to study alveolar macrophage (AM) heterogeneity. The gradient was made with nine bovine albumin concentrations (from 8 to 30%). After centrif...