Assay of somatomedin C by cartridge extraction prior to radioimmunoassay with antiserum developed against synthetic somatomedin C

• Published in: Annals of clinical and laboratory science Vol. 18; no. 2; pp. 120 - 130
• Main Authors: PAI CHIH KAO, TATEISHI, K, ABBOUD, C. F, ZIMMERMAN, D, RANDALL, R. V, CHOH HAO LI
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA Institute for Clinical Science 01.03.1988
• Subjects: Adolescent; Adult; Age Factors; Aminoacids, peptides. Hormones. Neuropeptides; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Carrier Proteins - blood; Child; Child, Preschool; Chromatography, Gel; Cross Reactions; Female; Fundamental and applied biological sciences. Psychology; Humans; Hydrogen-Ion Concentration; Immune Sera; Infant; Insulin-Like Growth Factor Binding Proteins; Insulin-Like Growth Factor I - blood; Insulin-Like Growth Factor I - chemical synthesis; Insulin-Like Growth Factor I - immunology; Male; Middle Aged; Proinsulin - blood; Protein Binding; Proteins; Radioimmunoassay - methods; Somatomedins - blood; Assay; Human; Binding protein; Purification; Somatomedin C; Radioimmunoassay; Clinical biology; Interference; Technique; Blood

The whole molecule of human somatomedin C (SM-C) prepared by the total synthesis method was used as an antigen to produce an antiserum for a radioimmunoassay. Since plasma proteins that bind SM-C interfere with the assay, a method was developed that uses acid dissociation followed by C-2 cartridge e...