Immunocytochemical staining of unstained versus previously stained cytologic preparations
Immunoperoxidase (IP) staining of fine needle aspiration specimens may provide useful adjunct information in cytodiagnosis. IP may be used on unstained direct smears or cell block material if available. In the absence of such material, IP is sometimes used on previously stained (Papanicolaou) smears...
Saved in:
Published in | Acta cytologica Vol. 39; no. 3; p. 379 |
---|---|
Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Switzerland
01.05.1995
|
Subjects | |
Online Access | Get more information |
Cover
Loading…
Abstract | Immunoperoxidase (IP) staining of fine needle aspiration specimens may provide useful adjunct information in cytodiagnosis. IP may be used on unstained direct smears or cell block material if available. In the absence of such material, IP is sometimes used on previously stained (Papanicolaou) smears. Few studies have examined the adequacy of such preparations, however. We compared IP staining results on unstained, alcohol-fixed or air-dried smears versus alcohol-fixed, previously Papanicolaou stained smears and versus alcohol-fixed, decolorized, Papanicolaou-stained smears, using antibodies to keratin (CAM 5.2, AE1/AE3 and K903), epithelial membrane antigen, monoclonal carcinoembryonic antigen, desmin, muscle-specific actin (HHF-35), vimentin, leukocyte common antigen, B and T cell markers L26 and UCHL-1, and prostate-specific antigen. Seventy-five sets of smears were made from various neoplastic or nonneoplastic tissues received as surgical specimens, and antibodies were selected for anticipated positive staining. The results were graded in a semiquantitative fashion. All the methods gave comparable results regarding expected positive staining, background staining and preservation of cellularity. We conclude that these methods of smear preparation are of equal utility in adjunctive immunocytochemical studies. |
---|---|
AbstractList | Immunoperoxidase (IP) staining of fine needle aspiration specimens may provide useful adjunct information in cytodiagnosis. IP may be used on unstained direct smears or cell block material if available. In the absence of such material, IP is sometimes used on previously stained (Papanicolaou) smears. Few studies have examined the adequacy of such preparations, however. We compared IP staining results on unstained, alcohol-fixed or air-dried smears versus alcohol-fixed, previously Papanicolaou stained smears and versus alcohol-fixed, decolorized, Papanicolaou-stained smears, using antibodies to keratin (CAM 5.2, AE1/AE3 and K903), epithelial membrane antigen, monoclonal carcinoembryonic antigen, desmin, muscle-specific actin (HHF-35), vimentin, leukocyte common antigen, B and T cell markers L26 and UCHL-1, and prostate-specific antigen. Seventy-five sets of smears were made from various neoplastic or nonneoplastic tissues received as surgical specimens, and antibodies were selected for anticipated positive staining. The results were graded in a semiquantitative fashion. All the methods gave comparable results regarding expected positive staining, background staining and preservation of cellularity. We conclude that these methods of smear preparation are of equal utility in adjunctive immunocytochemical studies. |
Author | Abendroth, C S Dabbs, D J |
Author_xml | – sequence: 1 givenname: C S surname: Abendroth fullname: Abendroth, C S organization: Department of Pathology, College of Medicine, Pennsylvania State University, Hershey, USA – sequence: 2 givenname: D J surname: Dabbs fullname: Dabbs, D J |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/7539200$$D View this record in MEDLINE/PubMed |
BookMark | eNo1j8tKAzEYhbOo1Lb6CEJeYCDXSbqU4qVQcKMLVyWT_KmRSTJMJoV5e6nW1eHw8R04a7RIOcECrQghtJFSqFu0LuWbEM7bli_RUkm-ZYSs0Oc-xpqynadsvyAGa3pcJhNSSCecPa7pt4HDZxhLLXgY4RxyLf2M_8lF7vMp2AsczGimkFO5Qzfe9AXur7lBH89P77vX5vD2st89HpqBcTk1XFGihLKt1lYa33EuKGgQ4JQ3TlJNZAeeA5OKbWXrFTdOESqEFJpZ2rENevjbHWoXwR2HMUQzzsfrRfYDYthQOg |
ContentType | Journal Article |
DBID | CGR CUY CVF ECM EIF NPM |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) |
DatabaseTitleList | MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | no_fulltext_linktorsrc |
Discipline | Medicine |
ExternalDocumentID | 7539200 |
Genre | Journal Article Comparative Study |
GroupedDBID | --- .GJ 0R~ 0~5 0~B 23M 3O. 4.4 53G 5GY 5RE 8UI AAQQT AAYIC ABJNI ABPAZ ACGFS ACNCT ACPSR ADBBV AEYAO AFJJK AI. ALDHI ALMA_UNASSIGNED_HOLDINGS AZPMC CAG CGR COF CUY CVF CYUIP E0A EBS ECM EIF EJD EMOBN F5P FB. HZ~ IY7 KUZGX L7B NPM O1H O9- RKO RXVBD SJN TSP UDS UJ6 VH1 WH7 ZGI ZXP ~02 |
ID | FETCH-LOGICAL-p235t-3710747c688c5afb3341e8e4ed7fad51805bef3e2572956f73ad701445482c1b2 |
ISSN | 0001-5547 |
IngestDate | Sat Sep 28 07:34:06 EDT 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 3 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-p235t-3710747c688c5afb3341e8e4ed7fad51805bef3e2572956f73ad701445482c1b2 |
PMID | 7539200 |
ParticipantIDs | pubmed_primary_7539200 |
PublicationCentury | 1900 |
PublicationDate | 1995-05-01 |
PublicationDateYYYYMMDD | 1995-05-01 |
PublicationDate_xml | – month: 05 year: 1995 text: 1995-05-01 day: 01 |
PublicationDecade | 1990 |
PublicationPlace | Switzerland |
PublicationPlace_xml | – name: Switzerland |
PublicationTitle | Acta cytologica |
PublicationTitleAlternate | Acta Cytol |
PublicationYear | 1995 |
SSID | ssj0033663 |
Score | 1.5710013 |
Snippet | Immunoperoxidase (IP) staining of fine needle aspiration specimens may provide useful adjunct information in cytodiagnosis. IP may be used on unstained direct... |
SourceID | pubmed |
SourceType | Index Database |
StartPage | 379 |
SubjectTerms | Biopsy, Needle Cytodiagnosis Evaluation Studies as Topic Female Humans Immunoenzyme Techniques Immunohistochemistry - methods Male Neoplasms - diagnosis Neoplasms - immunology Neoplasms - pathology Staining and Labeling - methods |
Title | Immunocytochemical staining of unstained versus previously stained cytologic preparations |
URI | https://www.ncbi.nlm.nih.gov/pubmed/7539200 |
Volume | 39 |
hasFullText | |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3JasMwEBVdaMmldAvd0aG34hJbtqUcQxfSQnJKID0FSZZOrROoU0i_vqPFC-lC24uxNLIx8-TxzHgWhC7BZuBdxtJApVQHpp1FwCVRASNchZlWna7tGTkYpv1x_DhJJnUzRZtdUohr-f5lXsl_UIU5wNVkyf4B2eqmMAHngC8cAWE4_grjB5PcMZPLwrS9cnn_Nh3KRzIvcjsCldLEXixeTUGANxPz-ry8KinmYiv-DNHVAS8deGVtWlnwelklxXtCmVoHzi3T8KDeciGcau7_OGU-xy6pA_gqMRkGoGfQpph0NYf8diANmUdcN5gGv-cvluFgCXVdHdKfiSsFrz1lHa1TZmTW0Phf3LeVkLTsjecesIW2_PIVs8CqB6NdtOP1etxzIO2hNZXvo-2Bj1w4QE-fscIlVnimcYUVdljhGitcUioQcBOrQzS-vxvd9APf1iKYRyQpQKSbIFgqU8ZkwrUgoEgopmKVUc2zJGSdRChNFAjTCKxXTQnPqDF8wbiMZCiiNtrIZ7k6QhgGkTTJ8IqJmESMs4gnREnCQTEWLD5GbceU6dzVLpl6bp18RzhFrXpHnKFNDa-KOge9qxAXFooPwUozqQ |
link.rule.ids | 786 |
linkProvider | National Library of Medicine |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Immunocytochemical+staining+of+unstained+versus+previously+stained+cytologic+preparations&rft.jtitle=Acta+cytologica&rft.au=Abendroth%2C+C+S&rft.au=Dabbs%2C+D+J&rft.date=1995-05-01&rft.issn=0001-5547&rft.volume=39&rft.issue=3&rft.spage=379&rft_id=info%3Apmid%2F7539200&rft_id=info%3Apmid%2F7539200&rft.externalDocID=7539200 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0001-5547&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0001-5547&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0001-5547&client=summon |