Electrostatic force microscopy: imaging DNA and protein polarizations one by one

We present electrostatic force microscopy images of double-stranded DNA and transcription complex on an insulating mica substrate obtained with molecular resolution using a frequency-mode noncontact atomic force microscope. The electrostatic potential images show that both DNA and transcription comp...

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Published inNanotechnology Vol. 20; no. 14; p. 145102
Main Authors Mikamo-Satoh, Eriko, Yamada, Fumihiko, Takagi, Akihiko, Matsumoto, Takuya, Kawai, Tomoji
Format Journal Article
LanguageEnglish
Published England 08.04.2009
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Abstract We present electrostatic force microscopy images of double-stranded DNA and transcription complex on an insulating mica substrate obtained with molecular resolution using a frequency-mode noncontact atomic force microscope. The electrostatic potential images show that both DNA and transcription complexes are polarized with an upward dipole moment. Potential differences of these molecules from the mica substrate enabled us to estimate dipole moments of isolated DNA and transcription complex in zero external field to be 0.027 D/base and 0.16 D/molecule, respectively. Scanning capacitance microscopy demonstrates characteristic contrast inversion between DNA and transcription complex images, indicating the difference in electric polarizability of these molecules. These findings indicate that the electrostatic properties of individual biological molecules can be imaged on an insulator substrate while retaining complex formation.
AbstractList We present electrostatic force microscopy images of double-stranded DNA and transcription complex on an insulating mica substrate obtained with molecular resolution using a frequency-mode noncontact atomic force microscope. The electrostatic potential images show that both DNA and transcription complexes are polarized with an upward dipole moment. Potential differences of these molecules from the mica substrate enabled us to estimate dipole moments of isolated DNA and transcription complex in zero external field to be 0.027 D/base and 0.16 D/molecule, respectively. Scanning capacitance microscopy demonstrates characteristic contrast inversion between DNA and transcription complex images, indicating the difference in electric polarizability of these molecules. These findings indicate that the electrostatic properties of individual biological molecules can be imaged on an insulator substrate while retaining complex formation.
Author Takagi, Akihiko
Kawai, Tomoji
Matsumoto, Takuya
Mikamo-Satoh, Eriko
Yamada, Fumihiko
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Snippet We present electrostatic force microscopy images of double-stranded DNA and transcription complex on an insulating mica substrate obtained with molecular...
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StartPage 145102
SubjectTerms DNA - chemistry
Image Processing, Computer-Assisted - methods
Microscopy, Atomic Force - methods
Proteins - chemistry
Static Electricity
United States
Title Electrostatic force microscopy: imaging DNA and protein polarizations one by one
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