Enhancement of cellular antioxidant-defence preserves diastolic dysfunction via regulation of both diastolic Zn2+ and Ca2+ and prevention of RyR2-leak in hyperglycemic cardiomyocytes

• Published in: Oxidative medicine and cellular longevity Vol. 2014; p. 290381
• Main Authors: Tuncay, Erkan, Okatan, Esma N, Toy, Aysegul, Turan, Belma
• Format: Journal Article
• Language: English
• Published: United States Hindawi Publishing Corporation 2014
• Subjects: Acetylcysteine - pharmacology; Acetylcysteine - therapeutic use; Animals; Antioxidants - metabolism; Blood Glucose - metabolism; Body Weight - drug effects; Calcium - metabolism; Diabetes Mellitus, Experimental - complications; Diabetes Mellitus, Experimental - metabolism; Diabetes Mellitus, Experimental - pathology; Diabetes Mellitus, Experimental - physiopathology; Diastole - drug effects; Heart Ventricles - drug effects; Heart Ventricles - pathology; Heart Ventricles - physiopathology; Hyperglycemia - complications; Hyperglycemia - metabolism; Hyperglycemia - pathology; Hyperglycemia - physiopathology; In Vitro Techniques; Intracellular Space - drug effects; Intracellular Space - metabolism; Male; Myocardium - metabolism; Myocardium - pathology; Myocardium - ultrastructure; Myocytes, Cardiac - drug effects; Myocytes, Cardiac - metabolism; Myocytes, Cardiac - pathology; Myocytes, Cardiac - ultrastructure; NF-kappa B - metabolism; Oxidation-Reduction - drug effects; Oxidative Stress - drug effects; Rats; Rats, Wistar; Ryanodine Receptor Calcium Release Channel - metabolism; Tacrolimus Binding Proteins - metabolism; Zinc - metabolism

We examined whether cellular antioxidant-defence enhancement preserves diastolic dysfunction via regulation of both diastolic intracellular free Zn(2+) and Ca(2+) levels ([Zn(2+)]i and [Ca(2+)]i) levels N-acetyl cysteine (NAC) treatment (4 weeks) of diabetic rats preserved altered cellular redox sta...