Transcriptomic Analysis of Alzheimer's Disease Pathways in a Pakistani Population
Alzheimer's disease (AD) is a multifactorial neurodegenerative disorder that is most prevalent in elderly individuals, especially in developed countries, and its prevalence is now increasing in developing countries like Pakistan. Our goal was to characterize key genes and their levels of expres...
Saved in:
Published in | JAD reports Vol. 8; no. 1; pp. 479 - 493 |
---|---|
Main Authors | , , , , , , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
01.01.2024
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Alzheimer's disease (AD) is a multifactorial neurodegenerative disorder that is most prevalent in elderly individuals, especially in developed countries, and its prevalence is now increasing in developing countries like Pakistan.
Our goal was to characterize key genes and their levels of expression and related molecular transcriptome networks associated with AD pathogenesis in a pilot case-control study in a Pakistani population.
To obtain the spectrum of molecular networks associated with pathogenesis in AD patients in Pakistan (comparing cases and controls), we used high-throughput qRT-PCR (TaqMan Low-Density Array;
= 33 subjects) coupled with Affymetrix Arrays (
= 8) and Ingenuity Pathway Analysis (IPA) to identify signature genes associated with Amyloid processing and disease pathways.
We confirmed 16 differentially expressed AD-related genes, including maximum fold changes observed in
and
. The global gene expression study observed that 61% and 39% of genes were significantly (
-value 0.05) up- and downregulated, respectively, in AD patients compared to healthy controls. The key pathways include, e.g.,
,
, and
. The top-scoring networks in Diseases and Disorders Development were
,
, and
.
Our pilot study offers a non-invasive and efficient way of investigating gene expression patterns by combining TLDA and global gene expression method in AD patients by utilizing whole blood. This provides valuable insights into the expression status of genes related to
, which could play potential role in future studies to identify sensitive, early biomarkers of AD in general. |
---|---|
AbstractList | BackgroundAlzheimer's disease (AD) is a multifactorial neurodegenerative disorder that is most prevalent in elderly individuals, especially in developed countries, and its prevalence is now increasing in developing countries like Pakistan.ObjectiveOur goal was to characterize key genes and their levels of expression and related molecular transcriptome networks associated with AD pathogenesis in a pilot case-control study in a Pakistani population.MethodsTo obtain the spectrum of molecular networks associated with pathogenesis in AD patients in Pakistan (comparing cases and controls), we used high-throughput qRT-PCR (TaqMan Low-Density Array; n = 33 subjects) coupled with Affymetrix Arrays (n = 8) and Ingenuity Pathway Analysis (IPA) to identify signature genes associated with Amyloid processing and disease pathways.ResultsWe confirmed 16 differentially expressed AD-related genes, including maximum fold changes observed in CAPNS2 and CAPN1. The global gene expression study observed that 61% and 39% of genes were significantly (p-value 0.05) up- and downregulated, respectively, in AD patients compared to healthy controls. The key pathways include, e.g., Amyloid Processing, Neuroinflammation Signaling, and ErbB4 Signaling. The top-scoring networks in Diseases and Disorders Development were Neurological Disease, Organismal Injury and Abnormalities, and Psychological Disorders.ConclusionsOur pilot study offers a non-invasive and efficient way of investigating gene expression patterns by combining TLDA and global gene expression method in AD patients by utilizing whole blood. This provides valuable insights into the expression status of genes related to Amyloid Processing, which could play potential role in future studies to identify sensitive, early biomarkers of AD in general. Alzheimer's disease (AD) is a multifactorial neurodegenerative disorder that is most prevalent in elderly individuals, especially in developed countries, and its prevalence is now increasing in developing countries like Pakistan. Our goal was to characterize key genes and their levels of expression and related molecular transcriptome networks associated with AD pathogenesis in a pilot case-control study in a Pakistani population. To obtain the spectrum of molecular networks associated with pathogenesis in AD patients in Pakistan (comparing cases and controls), we used high-throughput qRT-PCR (TaqMan Low-Density Array; = 33 subjects) coupled with Affymetrix Arrays ( = 8) and Ingenuity Pathway Analysis (IPA) to identify signature genes associated with Amyloid processing and disease pathways. We confirmed 16 differentially expressed AD-related genes, including maximum fold changes observed in and . The global gene expression study observed that 61% and 39% of genes were significantly ( -value 0.05) up- and downregulated, respectively, in AD patients compared to healthy controls. The key pathways include, e.g., , , and . The top-scoring networks in Diseases and Disorders Development were , , and . Our pilot study offers a non-invasive and efficient way of investigating gene expression patterns by combining TLDA and global gene expression method in AD patients by utilizing whole blood. This provides valuable insights into the expression status of genes related to , which could play potential role in future studies to identify sensitive, early biomarkers of AD in general. |
Author | Moses, Gemeyel Jana, Siddhartha S Chandra, Vijay Korba, Brent E Loffredo, Christopher A Cotin, Sharleine T Johnson, Jheannelle Bhatti, Attya Mondal, Tanmoy Noreen, Zarish Nnanabu, Thomas Quartey, Ruth Shahzad, Sharoon Clark, Marika Kwabi-Addo, Bernard Howell, Charles D Bhatti, Muhammad Farrukh Ghosh, Somiranjan Nunlee-Bland, Gail |
Author_xml | – sequence: 1 givenname: Tanmoy surname: Mondal fullname: Mondal, Tanmoy organization: Department of Biology, Howard University, Washington, DC, USA – sequence: 2 givenname: Zarish surname: Noreen fullname: Noreen, Zarish organization: Department of Healthcare Biotechnology, National University of Sciences and Technology (NUST), Islamabad, Pakistan – sequence: 3 givenname: Christopher A surname: Loffredo fullname: Loffredo, Christopher A organization: Department of Oncology, Georgetown University, Washington, DC, USA – sequence: 4 givenname: Jheannelle surname: Johnson fullname: Johnson, Jheannelle organization: Department of Biology, Howard University, Washington, DC, USA – sequence: 5 givenname: Attya surname: Bhatti fullname: Bhatti, Attya organization: Department of Healthcare Biotechnology, National University of Sciences and Technology (NUST), Islamabad, Pakistan – sequence: 6 givenname: Gail surname: Nunlee-Bland fullname: Nunlee-Bland, Gail organization: Department of Pediatrics and Child Health, College of Medicine, Howard University, Washington, DC, USA – sequence: 7 givenname: Ruth surname: Quartey fullname: Quartey, Ruth organization: Department of Internal Medicine, College of Medicine, Howard University, Washington, DC, USA – sequence: 8 givenname: Charles D surname: Howell fullname: Howell, Charles D organization: Department of Internal Medicine, College of Medicine, Howard University, Washington, DC, USA – sequence: 9 givenname: Gemeyel surname: Moses fullname: Moses, Gemeyel organization: Department of Biology, Howard University, Washington, DC, USA – sequence: 10 givenname: Thomas surname: Nnanabu fullname: Nnanabu, Thomas organization: Department of Biology, Howard University, Washington, DC, USA – sequence: 11 givenname: Sharleine T surname: Cotin fullname: Cotin, Sharleine T organization: Department of Biology, Howard University, Washington, DC, USA – sequence: 12 givenname: Marika surname: Clark fullname: Clark, Marika organization: Department of Biology, Howard University, Washington, DC, USA – sequence: 13 givenname: Vijay surname: Chandra fullname: Chandra, Vijay organization: Department of Neurology, Primus Hospital, New Delhi, India – sequence: 14 givenname: Siddhartha S surname: Jana fullname: Jana, Siddhartha S organization: Laboratory of Molecular and Cellular Biology (LMCB), Indian Association for the Cultivation of Science, Kolkata, India – sequence: 15 givenname: Bernard surname: Kwabi-Addo fullname: Kwabi-Addo, Bernard organization: Department of Biochemistry, College of Medicine, Howard University, Washington, DC, USA – sequence: 16 givenname: Brent E surname: Korba fullname: Korba, Brent E organization: Department of Microbiology and Immunology, Georgetown University, Washington, DC, USA (retired, May 2022) – sequence: 17 givenname: Sharoon surname: Shahzad fullname: Shahzad, Sharoon organization: Primary and Secondary Healthcare Department, Basic Health Unit, Union council Mundekey, Kasur, Pakistan – sequence: 18 givenname: Muhammad Farrukh surname: Bhatti fullname: Bhatti, Muhammad Farrukh organization: Department of Medicine, Pakistan Institute of Medical Sciences, Islamabad, Pakistan – sequence: 19 givenname: Somiranjan surname: Ghosh fullname: Ghosh, Somiranjan organization: Department of Pediatrics and Child Health, College of Medicine, Howard University, Washington, DC, USA |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/38549628$$D View this record in MEDLINE/PubMed |
BookMark | eNo1kE1Lw0AURQdRbK3d-ANkdrqJzryXmUyWwfoFBavUdXhNJnQ0mcRMgtRfb8G6ulw4nAv3jB371lvGLqS4QUC8zRZvEaCQsT5iU1AxRLEBnLB5CB9CCGlAa8BTNkGj4lSDmbLXdU8-FL3rhrZxBc881bvgAm8rntU_W-sa218FvnDBUrB8RcP2m3aBO89p3z5dGMg7vmq7sabBtf6cnVRUBzs_5Iy9P9yv756i5cvj8122jDqQcoiSMlXaVAkhVqpUikooTJqIpJRxqTWCqUipSlJKFipAnRpUVsnUgCiM3uCMXf95u779Gm0Y8saFwtY1eduOIUcBsF_QcbxHLw_ouGlsmXe9a6jf5f834C9XMF3o |
ContentType | Journal Article |
Copyright | 2024 – The authors. Published by IOS Press. |
Copyright_xml | – notice: 2024 – The authors. Published by IOS Press. |
DBID | NPM 7X8 |
DOI | 10.3233/ADR-230146 |
DatabaseName | PubMed MEDLINE - Academic |
DatabaseTitle | PubMed MEDLINE - Academic |
DatabaseTitleList | MEDLINE - Academic PubMed |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
EISSN | 2542-4823 |
EndPage | 493 |
ExternalDocumentID | 38549628 |
Genre | Journal Article |
GroupedDBID | ACGFS ACPQW ADBBV ADZMO ALMA_UNASSIGNED_HOLDINGS AOIJS EBS EJD GROUPED_DOAJ HYE IOS M~E NPM OK1 RPM 7X8 PGMZT |
ID | FETCH-LOGICAL-p211t-7d9568f7a33f5d55ad2c89707d14d66328fa55f1a9ae2f2369835e519820c86b3 |
IngestDate | Sat Aug 17 05:36:43 EDT 2024 Thu May 23 23:26:53 EDT 2024 |
IsDoiOpenAccess | false |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 1 |
Keywords | pathogenesis amyloid processing Alzheimer’s disease transcriptomic gene expression |
Language | English |
License | 2024 – The authors. Published by IOS Press. |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-p211t-7d9568f7a33f5d55ad2c89707d14d66328fa55f1a9ae2f2369835e519820c86b3 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
PMID | 38549628 |
PQID | 3022568644 |
PQPubID | 23479 |
PageCount | 15 |
ParticipantIDs | proquest_miscellaneous_3022568644 pubmed_primary_38549628 |
PublicationCentury | 2000 |
PublicationDate | 2024-01-01 |
PublicationDateYYYYMMDD | 2024-01-01 |
PublicationDate_xml | – month: 01 year: 2024 text: 2024-01-01 day: 01 |
PublicationDecade | 2020 |
PublicationPlace | Netherlands |
PublicationPlace_xml | – name: Netherlands |
PublicationTitle | JAD reports |
PublicationTitleAlternate | J Alzheimers Dis Rep |
PublicationYear | 2024 |
SSID | ssj0001826623 |
Score | 2.2877064 |
Snippet | Alzheimer's disease (AD) is a multifactorial neurodegenerative disorder that is most prevalent in elderly individuals, especially in developed countries, and... BackgroundAlzheimer's disease (AD) is a multifactorial neurodegenerative disorder that is most prevalent in elderly individuals, especially in developed... |
SourceID | proquest pubmed |
SourceType | Aggregation Database Index Database |
StartPage | 479 |
Title | Transcriptomic Analysis of Alzheimer's Disease Pathways in a Pakistani Population |
URI | https://www.ncbi.nlm.nih.gov/pubmed/38549628 https://search.proquest.com/docview/3022568644 |
Volume | 8 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1dS9xAFB3s-tKXomhbPxlB8KFM685HMnlcXEVkXduShaUvYZKZYRc0EXdF9Nd7Z_K1axVsX0IIZAg5kzvn3sk9B6HDMNMijXhAbCAs4dQqEilGCdXcci2PWeT9Uy6HwfmIX4zFuDXj9N0l8_R79vRqX8n_oArXAFfXJfsPyDaDwgU4B3zhCAjD8X0Yu4XGf_aut3hJYKR3_TQxU2-NEs6cxqbbhnGC_JMH9ej_gVU1e8yn3342Nl5LZLXXr_cUGlwKSOF91ThW-U3R1OOHxV3lcP_HuRo2NeZB4RrFdPFCx6CtoFYOXX4ywbrg_7oxi6UIyhdKEcaHLMg2KeGy7CCu46v8axqVsZKXLjLVsstLo8SXEZ1R5pUl-r8JddnfK7LZw6vkbDQYJPHpOP6AVmkYiXAht_bFNkiigOeV8rRuyB_tgG-nFp5ixGvoU5Ub4F4J9DpaMfkG-rUMMq5BxoXFDchHM1xBjGuI8TTHCjcQ4xbiTTQ6O41Pzknlg0FuIT2fk1C7nk4bKsas0EIoTTMZhceh7nINjJFKq4SwXSe0Ti1lQQS02gA1B3aXySBln1EnL3LzFWEbSJVyIaRinCspoy6lBjJeJbI0TQXdQgf1y0ggzrjNI5Wb4n6WMCB78BRAn7fQl_ItJbelIErCpOBRQOX2O-7eQR_bqbOLOvO7e7MHvG6e7nvMngF5F1J0 |
link.rule.ids | 315,786,790,870,27957,27958 |
linkProvider | Directory of Open Access Journals |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Transcriptomic+Analysis+of+Alzheimer%27s+Disease+Pathways+in+a+Pakistani+Population&rft.jtitle=JAD+reports&rft.au=Mondal%2C+Tanmoy&rft.au=Noreen%2C+Zarish&rft.au=Loffredo%2C+Christopher+A&rft.au=Johnson%2C+Jheannelle&rft.date=2024-01-01&rft.eissn=2542-4823&rft.volume=8&rft.issue=1&rft.spage=479&rft.epage=493&rft_id=info:doi/10.3233%2FADR-230146&rft.externalDBID=NO_FULL_TEXT |