Programmed cell death-1 in patients with primary liver cancer and its effect on prognosis

• Published in: Journal of B.U.ON. : official journal of the Balkan Union of Oncology Vol. 24; no. 3; p. 1167
• Main Authors: Yang, Min, Dou, Wen Wen, Sun, Guang Hong, Zhang, Yan Lan, Su, Yan, Xie, Rui Zhu
• Format: Journal Article
• Language: English
• Published: Greece 01.05.2019
• Subjects: Adult; Aged; Case-Control Studies; Female; Hepatitis B, Chronic - blood; Humans; Liver Neoplasms - blood; Liver Neoplasms - genetics; Liver Neoplasms - immunology; Liver Neoplasms - pathology; Male; Middle Aged; Prognosis; Programmed Cell Death 1 Receptor - biosynthesis; Programmed Cell Death 1 Receptor - blood; Programmed Cell Death 1 Receptor - genetics; Programmed Cell Death 1 Receptor - immunology; RNA, Messenger - biosynthesis; RNA, Messenger - blood; RNA, Messenger - genetics; T-Lymphocytes - pathology
• ISSN: 2241-6293

To detect the expression of programmed cell death-1 (PD-1) in peripheral blood T lymphocytes of patients with primary liver cancer and its effect on their prognosis. The medical records of 42 patients with primary liver cancer, 36 patients with chronic hepatitis B (CHB) and 38 healthy volunteers composed the liver cancer group, benign lesion group and control group, respectively. Fluorescence quantitative RT-PCR (qRT-PCR) was used for detecting the expression level of PD-1 mRNA in peripheral blood T lymphocytes of subjects in the three groups, and flow cytometry was used for detecting the positive expression of PD-l protein on the surface of T lymphocytes of subjects. Patients in the control group and the benign lesion group had lower expression level of PD-1 mRNA than those in the liver cancer group (p<0.05). Patients in the control group had lower expression level of PD-1 mRNA than those in the benign lesion group (p<0.05). Patients in the control group and the benign lesion group had lower positive expression rate of PD-1 protein on the surface of T lymphocytes than those in the liver cancer group (p<0.05). The expression of PD-1 in the peripheral blood was higher in patients with primary liver cancer. Patients in the PD-1 low expression group had significantly better prognosis than those in the PD-1 high expression group. PD-1 may be related to the occurrence and development of primary liver cancer and is worthy of further study.