Esterase Inhibition and Copper Release from Copper Iodide Dental Adhesives - An In Vitro Study
To investigate whether dental adhesives modified with polyacrylic acid copper iodide particles could inhibit esterase activity in vitro and the copper release rate from resin matrices, as well as the correlation between the two variables. Different concentrations of copper iodide (0.1, 0.5 and 1.0 m...
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Published in | The journal of adhesive dentistry Vol. 22; no. 3; p. 265 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Germany
01.05.2020
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Abstract | To investigate whether dental adhesives modified with polyacrylic acid copper iodide particles could inhibit esterase activity in vitro and the copper release rate from resin matrices, as well as the correlation between the two variables.
Different concentrations of copper iodide (0.1, 0.5 and 1.0 mg/ml) were incorporated into three commercially available adhesives representative of each type. Disk specimens (n = 3) were fabricated and incubated in cholesterol esterase and pseudo-cholinesterase solutions for 16 days (37°C, pH 7.0). The enzymatic activity and rate of copper release from resin matrices were evaluated at different 4, 8, 12, and 16 days with a UV/visible-light spectrophotometer.
Increased copper release and reduced enzymatic activity were observed with higher concentrations of copper iodide (p < 0.001). Greater copper release with reduced enzymatic activity was also demonstrated at the earlier time periods with this relationship reversing over time (p < 0.001). A moderate negative correlation between the variables was evident (-0.441; p = 0.01).
Adhesives containing copper iodide can inhibit esterase activity in a dose- and time-dependent manner. The correlation between the variables suggests that enzymatic activity may depend on the availability of copper. |
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AbstractList | To investigate whether dental adhesives modified with polyacrylic acid copper iodide particles could inhibit esterase activity in vitro and the copper release rate from resin matrices, as well as the correlation between the two variables.
Different concentrations of copper iodide (0.1, 0.5 and 1.0 mg/ml) were incorporated into three commercially available adhesives representative of each type. Disk specimens (n = 3) were fabricated and incubated in cholesterol esterase and pseudo-cholinesterase solutions for 16 days (37°C, pH 7.0). The enzymatic activity and rate of copper release from resin matrices were evaluated at different 4, 8, 12, and 16 days with a UV/visible-light spectrophotometer.
Increased copper release and reduced enzymatic activity were observed with higher concentrations of copper iodide (p < 0.001). Greater copper release with reduced enzymatic activity was also demonstrated at the earlier time periods with this relationship reversing over time (p < 0.001). A moderate negative correlation between the variables was evident (-0.441; p = 0.01).
Adhesives containing copper iodide can inhibit esterase activity in a dose- and time-dependent manner. The correlation between the variables suggests that enzymatic activity may depend on the availability of copper. |
Author | Sabatini, Camila Fernandes, Gabriela Renné, Walter G Visser, Michelle B |
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BackLink | https://www.ncbi.nlm.nih.gov/pubmed/32435767$$D View this record in MEDLINE/PubMed |
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Keywords | saliva resins drug delivery copper iodide esterases adhesives |
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Snippet | To investigate whether dental adhesives modified with polyacrylic acid copper iodide particles could inhibit esterase activity in vitro and the copper release... |
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SubjectTerms | Copper Dental Cements Esterases Iodides Materials Testing |
Title | Esterase Inhibition and Copper Release from Copper Iodide Dental Adhesives - An In Vitro Study |
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