Imine Reductases, Reductive Aminases, and Amine Oxidases for the Synthesis of Chiral Amines: Discovery, Characterization, and Synthetic Applications

• Published in: Methods in enzymology Vol. 608; p. 131
• Main Authors: Cosgrove, Sebastian C, Brzezniak, Agata, France, Scott P, Ramsden, Jeremy I, Mangas-Sanchez, Juan, Montgomery, Sarah L, Heath, Rachel S, Turner, Nicholas J
• Format: Journal Article
• Language: English
• Published: United States 2018
• Subjects: Amines - chemistry; Amines - metabolism; Aminohydrolases - genetics; Aminohydrolases - metabolism; Aspergillus niger - enzymology; Aspergillus niger - genetics; Aspergillus niger - metabolism; Bacteria - enzymology; Bacteria - genetics; Bacteria - metabolism; Biocatalysis; Escherichia coli - enzymology; Escherichia coli - genetics; Escherichia coli - metabolism; Fungi - enzymology; Fungi - genetics; Fungi - metabolism; Imines - chemistry; Imines - metabolism; Monoamine Oxidase - genetics; Monoamine Oxidase - metabolism; Oxidation-Reduction; Oxidoreductases - genetics; Oxidoreductases - metabolism; Protein Engineering - methods; Stereoisomerism; Streptomyces - enzymology; Streptomyces - genetics; Streptomyces - metabolism; Synthetic Biology - methods; Imine reductase; In vivo and in vitro cascade reactions; Amine oxidase library screening; Reductive aminase; Amine oxidase; Biotransformations

Synthesis of the chiral amine moiety is a key challenge for synthetic organic chemistry due to its prevalence in many biologically active molecules. Imine reductase and amine oxidase enzymes have enabled the biocatalytic synthesis of a host of chiral amine compounds. In this chapter, procedures for...