Research on the Spectrophotometry for Determination of Flavonoids in Waste Residue of Stevia Rebaudiana

• Published in: Guang pu xue yu guang pu fen xi Vol. 35; no. 10; p. 2820
• Main Authors: Luo, Xu-yan, Fang, Hao-bin, Huang, Rui-yu, Zhao, Dong-fang, Zhu, Ting, Guo, Yang-yang, Zeng Yi-ping, Liu, Ren-yue, Zhu, Dong
• Format: Journal Article
• Language: Chinese
• Published: China 01.10.2015
• Subjects: Aluminum Compounds; Chlorides; Flavonoids; Indicators and Reagents; Reproducibility of Results; Rutin; Spectrophotometry; Stevia; Temperature
• ISSN: 1000-0593
• DOI: 10.3964/j.issn.1000-0593(2015)10-2820-05

A spectrophotometric method for the determination of flavonoids content in Waste Residue of Stevia Rebaudiana was established by comparing the UV-Vis spectra with three spectrophotometric methods: Direct, AlCl3 and NaNO2-Al (NO3)3-NaOH method. The effects of spectrophotometric reaction were studied by analyzing the wavelength, the dosage of color development reagent, reaction time, temperature and pH. The further research was studied by analyzing linearity between the absorbance and the concentration, the stability of this color system, the recovery rate and so on. The results showed that NaNO2-Al(NO3)3-NaOH method was best. The wavelength was 500 nm. The standard sample was rutin. There was a good linear relationship in the ranges of 2 - 24 μg x mL(-1), correlation coefficient R2 = 0.999 5. The recovery of the additional sample was 96.0%-104.3%. The relative standard deviation(RSD) is respectively 3.75%. The dosages of NaNO2 and Al(NO3)3 both were 1.0 mL, both of their reaction time was 6 min. The dosage of