Generation of Induced Pluripotent Stem Cell-Like Lines from Human Pancreatic Ductal Adenocarcinoma

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 1882; p. 33
• Main Authors: Kim, Jungsun, Zaret, Kenneth S
• Format: Journal Article
• Language: English
• Published: United States 2019
• Subjects: Animals; Carcinoma, Pancreatic Ductal - pathology; Carcinoma, Pancreatic Ductal - therapy; Cell Culture Techniques - instrumentation; Cell Culture Techniques - methods; Cell Differentiation - genetics; Cell Line, Tumor; Cellular Reprogramming Techniques - methods; Fibroblasts; Genetic Vectors - genetics; HEK293 Cells; Humans; Induced Pluripotent Stem Cells - pathology; Lentivirus - genetics; Mice; Pancreatic Neoplasms - pathology; Pancreatic Neoplasms - therapy; Transcription Factors - genetics; Transduction, Genetic - instrumentation; Transduction, Genetic - methods; Xenograft Model Antitumor Assays - methods; Induced pluripotent stem cell; Pancreatic ductal adenocarcinoma; Pancreatic intraepithelial neoplasia

Pancreatic ductal adenocarcinoma (PDAC) has a dismal prognosis, mainly because the tumors are detected too late for effective treatment or for developing suitable therapeutics. Reprogramming cancer cells to pluripotency by induced pluripotent stem cell (iPSC) technology, which can be then programmed...