Inefficient processing of mRNA for the membrane form of IgE is a genetic mechanism to limit recruitment of IgE-secreting cells
Immunoglobulin E (IgE) is the key effector element in allergic diseases ranging from innocuous hay fever to life-threatening anaphylactic shock. Compared to other Ig classes, IgE serum levels are very low. In its membrane-bound form (mIgE), IgE behaves as a classical antigen receptor on B lymphocyte...
Saved in:
Published in | European journal of immunology Vol. 36; no. 7; pp. 1917 - 1925 |
---|---|
Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Germany
01.07.2006
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Immunoglobulin E (IgE) is the key effector element in allergic diseases ranging from innocuous hay fever to life-threatening anaphylactic shock. Compared to other Ig classes, IgE serum levels are very low. In its membrane-bound form (mIgE), IgE behaves as a classical antigen receptor on B lymphocytes. Expression of mIgE is essential for subsequent recruitment of IgE-secreting cells. We show that in activated, mIgE-bearing B cells, mRNA for the membrane forms of both murine and human epsilon (epsilon) heavy chains (HC) are poorly expressed compared to mRNA for the secreted forms. In contrast, in mIgG-bearing B cells, mRNA for the membrane forms of murine gamma-1 (gamma1) and the corresponding human gamma4 HC are expressed at a much higher level than mRNA for the respective secreted forms. We show that these findings correlate with the presence of deviant polyadenylation signal hexamers in the 3' untranslated region (UTR) of both murine and human epsilon genes, causing inefficient processing of primary transcripts and thus poor expression of the proteins and poor recruitment of IgE-producing cells in the immune response. Thus, we have identified a genetic steering mechanism in the regulation of IgE synthesis that represents a further means to restrain potentially dangerous, high serum IgE levels. |
---|---|
AbstractList | Immunoglobulin E (IgE) is the key effector element in allergic diseases ranging from innocuous hay fever to life-threatening anaphylactic shock. Compared to other Ig classes, IgE serum levels are very low. In its membrane-bound form (mIgE), IgE behaves as a classical antigen receptor on B lymphocytes. Expression of mIgE is essential for subsequent recruitment of IgE-secreting cells. We show that in activated, mIgE-bearing B cells, mRNA for the membrane forms of both murine and human epsilon (epsilon) heavy chains (HC) are poorly expressed compared to mRNA for the secreted forms. In contrast, in mIgG-bearing B cells, mRNA for the membrane forms of murine gamma-1 (gamma1) and the corresponding human gamma4 HC are expressed at a much higher level than mRNA for the respective secreted forms. We show that these findings correlate with the presence of deviant polyadenylation signal hexamers in the 3' untranslated region (UTR) of both murine and human epsilon genes, causing inefficient processing of primary transcripts and thus poor expression of the proteins and poor recruitment of IgE-producing cells in the immune response. Thus, we have identified a genetic steering mechanism in the regulation of IgE synthesis that represents a further means to restrain potentially dangerous, high serum IgE levels. |
Author | Lamers, Marinus C Achatz-Straussberger, Gertrude Karnowski, Alexander Klockenbusch, Cordula Achatz, Gernot |
Author_xml | – sequence: 1 givenname: Alexander surname: Karnowski fullname: Karnowski, Alexander organization: Metschnikoff Laboratory, Max-Planck-Institut für Immunbiologie, Freiburg, Germany – sequence: 2 givenname: Gertrude surname: Achatz-Straussberger fullname: Achatz-Straussberger, Gertrude – sequence: 3 givenname: Cordula surname: Klockenbusch fullname: Klockenbusch, Cordula – sequence: 4 givenname: Gernot surname: Achatz fullname: Achatz, Gernot – sequence: 5 givenname: Marinus C surname: Lamers fullname: Lamers, Marinus C |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/16783846$$D View this record in MEDLINE/PubMed |
BookMark | eNo1kL1PwzAQxT0U0Q8YWZEnthTbiWN7rKpSKlUgIZgjx7m0rmKnxM7Awt9OopbppHe_e3fv5mjiWw8IPVCypISwZzjZJSOEpzxTfIJmhNAsYUqSKZqHcCKEqJyrWzSluZCpzPIZ-t15qGtrLPiIz11rIATrD7itsft4W-G67XA8Anbgyk57GAU3dneHDbYBa3wAD9GagTBH7W1wOLa4sc5G3IHpehvd6H0ZScIgDfiwwUDThDt0U-smwP21LtDXy-Zz_Zrs37e79WqfnBlRMSlNqXhJq0qUucioMYJpBpBmUBkqK5bmmaQ5F5UWGiQDoZRkVPGKp7XUoNMFerr4DhG_ewixcDaMFwyR2j4UuRRUcqEG8PEK9qWDqjh31unup_j_WPoHRstung |
ContentType | Journal Article |
DBID | CGR CUY CVF ECM EIF NPM 7X8 |
DOI | 10.1002/eji.200535495 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
DatabaseTitleList | MEDLINE MEDLINE - Academic |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Medicine Biology |
EndPage | 1925 |
ExternalDocumentID | 16783846 |
Genre | Research Support, Non-U.S. Gov't Journal Article |
GroupedDBID | --- .3N .55 .GA .GJ .HR .Y3 05W 0R~ 10A 1L6 1OB 1OC 1ZS 24P 31~ 33P 3O- 3SF 3WU 4.4 4ZD 50Y 50Z 51W 51X 52M 52N 52O 52P 52S 52T 52U 52W 52X 53G 5GY 5RE 5VS 66C 702 7PT 8-0 8-1 8-3 8-4 8-5 8UM 930 A03 AAESR AAEVG AAHHS AANLZ AAONW AASGY AAXRX AAZKR ABCQN ABCUV ABEFU ABEML ABIJN ABJNI ABLJU ABPVW ABQWH ACAHQ ACBWZ ACCFJ ACCZN ACFBH ACGFS ACPOU ACPRK ACSCC ACXBN ACXQS ADBBV ADEOM ADIZJ ADKYN ADMGS ADOZA ADXAS ADZMN ADZOD AEEZP AEIGN AEIMD AENEX AEQDE AEUQT AEUYR AFBPY AFFNX AFFPM AFGKR AFPWT AFRAH AFZJQ AHBTC AHMBA AI. AITYG AIURR AIWBW AJBDE AJXKR ALAGY ALMA_UNASSIGNED_HOLDINGS ALUQN AMBMR AMYDB AOETA ASPBG ATUGU AUFTA AVWKF AZBYB AZFZN AZVAB BAFTC BDRZF BFHJK BHBCM BMNLL BMXJE BNHUX BROTX BRXPI BY8 CGR CS3 CUY CVF D-E D-F DCZOG DPXWK DR2 DRFUL DRSTM DU5 EBD EBS ECM EIF EJD EMOBN F00 F01 F04 F5P FEDTE G-S G.N GNP GODZA H.T H.X HBH HF~ HGLYW HHY HHZ HVGLF HZ~ IX1 J0M J5H JPC KQQ L7B LATKE LAW LC2 LC3 LEEKS LH4 LITHE LOXES LP6 LP7 LUTES LW6 LYRES M65 MEWTI MK4 MRFUL MRSTM MSFUL MSSTM MXFUL MXSTM N04 N05 N9A NF~ NNB NPM O66 O9- OHT OIG OK1 OVD P2P P2W P2X P4D PALCI PQQKQ Q.N Q11 QB0 QRW R.K RGB RIWAO RJQFR ROL RWI RX1 SAMSI SUPJJ SV3 TEORI UB1 UPT V2E VH1 W8V W99 WBKPD WHWMO WIB WIH WIK WIN WJL WOHZO WQJ WRC WUP WVDHM WXSBR X7M XG1 XPP XV2 Y6R ZGI ZXP ZZTAW ~IA ~KM ~WT 7X8 AAMNL |
ID | FETCH-LOGICAL-p209t-bcb95b1dd7b6741cc72a2ee34edc18d236481657da7ae82e79982195d53f8aea3 |
ISSN | 0014-2980 |
IngestDate | Wed Dec 04 02:11:05 EST 2024 Sat Sep 28 07:42:36 EDT 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 7 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-p209t-bcb95b1dd7b6741cc72a2ee34edc18d236481657da7ae82e79982195d53f8aea3 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
PMID | 16783846 |
PQID | 68718579 |
PQPubID | 23479 |
PageCount | 9 |
ParticipantIDs | proquest_miscellaneous_68718579 pubmed_primary_16783846 |
PublicationCentury | 2000 |
PublicationDate | 2006-Jul 20060701 |
PublicationDateYYYYMMDD | 2006-07-01 |
PublicationDate_xml | – month: 07 year: 2006 text: 2006-Jul |
PublicationDecade | 2000 |
PublicationPlace | Germany |
PublicationPlace_xml | – name: Germany |
PublicationTitle | European journal of immunology |
PublicationTitleAlternate | Eur J Immunol |
PublicationYear | 2006 |
SSID | ssj0009659 |
Score | 2.10653 |
Snippet | Immunoglobulin E (IgE) is the key effector element in allergic diseases ranging from innocuous hay fever to life-threatening anaphylactic shock. Compared to... |
SourceID | proquest pubmed |
SourceType | Aggregation Database Index Database |
StartPage | 1917 |
SubjectTerms | Animals Antibody-Producing Cells - cytology Antibody-Producing Cells - immunology Antibody-Producing Cells - metabolism Cell Line, Tumor Cell Movement - genetics Cell Movement - immunology Cells, Cultured Humans Immunoglobulin E - biosynthesis Immunoglobulin E - genetics Immunoglobulin E - metabolism Mice Mice, Knockout Receptors, IgE - deficiency Receptors, IgE - genetics RNA Processing, Post-Transcriptional - immunology RNA, Messenger - metabolism |
Title | Inefficient processing of mRNA for the membrane form of IgE is a genetic mechanism to limit recruitment of IgE-secreting cells |
URI | https://www.ncbi.nlm.nih.gov/pubmed/16783846 https://search.proquest.com/docview/68718579 |
Volume | 36 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwELaWIhAXBOVVnj5wQ4F1Xk6OVSkUqvZQtVJvKyeeVAtNstokQvTAv-F_MmM72WzVlYBLtIof8WY-2TOTmW8YewvFFGQSFZ6fiswLxVR7WYFWq4qhwL1Qi8Aw3hwdxwdn4dfz6Hwy-T2KWura7H1-dWNeyf9IFe-hXClL9h8kO0yKN_A3yhevKGG8_pWMv1RgGCDoe_7CRvy7IOby5Hh3iCAsoUSbuDL03qXJHbnYp0LmiuonUxIj9qAEYKqXgaroJeU8vcOdcNnN2z5WAId4DamYJkya3P3NRqd-z0RBqSdrXvtDtazqH65S9pBaM2AO19BeecSX2zUNxZ1ZOH2GZbvs9IDAQzx_vxNlQ2PLWO2h_dxdqvVp3MCqbq_7NWTv13B7tQgRQbbOU79XW7IUh0k52njJ7LzxRLAMs_BtbvxnAZrD0bgfCnRRGngIPLaDJLzGy20NJdd0i90mvkUq0fDxZMVSRpyM9sC3C3ZUrvjkD2vPJYJaN9Nme8boNacP2H1nkPBdi66HbALVNrtjS5T-3GZ3j1zwxSP2awQ3voIbrwtOcOOILo5w4z3c6EZJrYgdPm-44g5ufIAbb2tu4MZHcHNDVnDjBm6P2dmn_dO9A8-V7_AW_jRtvSzP0igTWsssRr01z6WvfIAgBJ2LRFPlgkTEkdRKKkh8kGj54_kZ6SgoEgUqeMK2qrqCZ4wHaAPGYYoKncDhscZOIvBRN81AQhhNd9ib_nXOcHukVeHfrLtmFieS2M7SHfbUvuXZwrK4zHpRPN_Y8oLdWwHzJdtCrMMrVEHb7LUBwB_RXYqO |
link.rule.ids | 314,780,784,27924,27925 |
linkProvider | Wiley-Blackwell |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Inefficient+processing+of+mRNA+for+the+membrane+form+of+IgE+is+a+genetic+mechanism+to+limit+recruitment+of+IgE-secreting+cells&rft.jtitle=European+journal+of+immunology&rft.au=Karnowski%2C+Alexander&rft.au=Achatz-Straussberger%2C+Gertrude&rft.au=Klockenbusch%2C+Cordula&rft.au=Achatz%2C+Gernot&rft.date=2006-07-01&rft.issn=0014-2980&rft.volume=36&rft.issue=7&rft.spage=1917&rft_id=info:doi/10.1002%2Feji.200535495&rft_id=info%3Apmid%2F16783846&rft.externalDocID=16783846 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0014-2980&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0014-2980&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0014-2980&client=summon |