Endonuclease restriction of SCAR amplicons SC811 is required to identify Ns-false-positive markers in PVS-susceptible potato cultivars

• Published in: Journal of applied genetics Vol. 49; no. 1; p. 45
• Main Authors: Szajko, Katarzyna, Chrzanowska, Mirosława, Strzelczyk-Zyta, Danuta, Zagórska, Helena, Marczewski, Waldemar
• Format: Journal Article
• Language: English
• Published: England 2008
• Subjects: Animals; Deoxyribonucleases, Type II Site-Specific - genetics; False Positive Reactions; Genetic Markers; Genetic Predisposition to Disease; Lycopersicon esculentum - genetics; Lycopersicon esculentum - virology; Plant Diseases - genetics; Plant Diseases - virology; Potyvirus - genetics; Potyvirus - pathogenicity; Random Amplified Polymorphic DNA Technique; Solanum tuberosum - genetics; Solanum tuberosum - virology

The Ns gene confers resistance of potato to Potato virus S (PVS). Sixteen German and Dutch potato cultivars, all registered in Poland, were found to be susceptible to PVS infection. However, scoring of the cultivars for the presence of the Ns-linked SCAR marker SC811(454) revealed additional amplico...