Endonuclease restriction of SCAR amplicons SC811 is required to identify Ns-false-positive markers in PVS-susceptible potato cultivars

The Ns gene confers resistance of potato to Potato virus S (PVS). Sixteen German and Dutch potato cultivars, all registered in Poland, were found to be susceptible to PVS infection. However, scoring of the cultivars for the presence of the Ns-linked SCAR marker SC811(454) revealed additional amplico...

Full description

Saved in:
Bibliographic Details
Published inJournal of applied genetics Vol. 49; no. 1; p. 45
Main Authors Szajko, Katarzyna, Chrzanowska, Mirosława, Strzelczyk-Zyta, Danuta, Zagórska, Helena, Marczewski, Waldemar
Format Journal Article
LanguageEnglish
Published England 2008
Subjects
Animals
Deoxyribonucleases, Type II Site-Specific - genetics
False Positive Reactions
Genetic Markers
Genetic Predisposition to Disease
Lycopersicon esculentum - genetics
Lycopersicon esculentum - virology
Plant Diseases - genetics
Plant Diseases - virology
Potyvirus - genetics
Potyvirus - pathogenicity
Random Amplified Polymorphic DNA Technique
Solanum tuberosum - genetics
Solanum tuberosum - virology
Online AccessGet full text

Cover

Abstract The Ns gene confers resistance of potato to Potato virus S (PVS). Sixteen German and Dutch potato cultivars, all registered in Poland, were found to be susceptible to PVS infection. However, scoring of the cultivars for the presence of the Ns-linked SCAR marker SC811(454) revealed additional amplicons with a similar electrophoretic migration rate as that of SC811(454), which resulted in ambiguous determination of the genotype at the Ns locus. MboI or FokI treatment of the PCR products allowed to detect their Ns-unspecificity in PVS-susceptible potato cultivars.
AbstractList The Ns gene confers resistance of potato to Potato virus S (PVS). Sixteen German and Dutch potato cultivars, all registered in Poland, were found to be susceptible to PVS infection. However, scoring of the cultivars for the presence of the Ns-linked SCAR marker SC811(454) revealed additional amplicons with a similar electrophoretic migration rate as that of SC811(454), which resulted in ambiguous determination of the genotype at the Ns locus. MboI or FokI treatment of the PCR products allowed to detect their Ns-unspecificity in PVS-susceptible potato cultivars.The Ns gene confers resistance of potato to Potato virus S (PVS). Sixteen German and Dutch potato cultivars, all registered in Poland, were found to be susceptible to PVS infection. However, scoring of the cultivars for the presence of the Ns-linked SCAR marker SC811(454) revealed additional amplicons with a similar electrophoretic migration rate as that of SC811(454), which resulted in ambiguous determination of the genotype at the Ns locus. MboI or FokI treatment of the PCR products allowed to detect their Ns-unspecificity in PVS-susceptible potato cultivars.
The Ns gene confers resistance of potato to Potato virus S (PVS). Sixteen German and Dutch potato cultivars, all registered in Poland, were found to be susceptible to PVS infection. However, scoring of the cultivars for the presence of the Ns-linked SCAR marker SC811(454) revealed additional amplicons with a similar electrophoretic migration rate as that of SC811(454), which resulted in ambiguous determination of the genotype at the Ns locus. MboI or FokI treatment of the PCR products allowed to detect their Ns-unspecificity in PVS-susceptible potato cultivars.
Author Strzelczyk-Zyta, Danuta
Szajko, Katarzyna
Chrzanowska, Mirosława
Zagórska, Helena
Marczewski, Waldemar
Author_xml – sequence: 1
  givenname: Katarzyna
  surname: Szajko
  fullname: Szajko, Katarzyna
  organization: Plant Breeding and Acclimatization Institute, Platanowa 19, 05-831 Młochów, Poland
– sequence: 2
  givenname: Mirosława
  surname: Chrzanowska
  fullname: Chrzanowska, Mirosława
– sequence: 3
  givenname: Danuta
  surname: Strzelczyk-Zyta
  fullname: Strzelczyk-Zyta, Danuta
– sequence: 4
  givenname: Helena
  surname: Zagórska
  fullname: Zagórska, Helena
– sequence: 5
  givenname: Waldemar
  surname: Marczewski
  fullname: Marczewski, Waldemar
BackLink https://www.ncbi.nlm.nih.gov/pubmed/18263969$$D View this record in MEDLINE/PubMed
BookMark eNo1kMtOwzAURL0oog_Y8AHIK3YBP9LYXpaqBaQKEAW2kWPfSIbUTuMEqT_Ad2NBWY1GOnM1c6do5IMHhC4ouaaEiJvbNeFUzVkuR2hCGc8zqiQfo2mMH4RwmQt2isZUsoKrQk3Q98rb4AfTgI6AO4h950zvgsehxtvl4gXrXds4E3xMVlKKXUzYfnAdWNwH7Cz43tUH_BizWjcRsjZE17svwDvdfUIXsfP4-X2bxSEaaHtXNYDb0OsUNkOTSN3FM3TyGz4_6gy9rVevy_ts83T3sFxsspYR1WeaWCKKquZKQzW3nGgjqKlkYazSEmpjNCmSEiMZY1oSoY0EMJQUXFQ25zN09Xe37cJ-SGvLnUutmkZ7CEMsBWFCiTlL4OURHKod2LLtXJpzKP8_x38AtUFyGA
ContentType Journal Article
DBID CGR
CUY
CVF
ECM
EIF
NPM
7X8
DOI 10.1007/BF03195248
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
MEDLINE - Academic
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
MEDLINE - Academic
DatabaseTitleList MEDLINE - Academic
MEDLINE
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Agriculture
ExternalDocumentID 18263969
Genre Journal Article
GroupedDBID ---
06D
0R~
0VY
203
29J
29~
2KG
2KM
2LR
2VQ
2WC
30V
3EA
4.4
406
408
53G
5GY
67N
8UJ
95.
96X
AACDK
AAHBH
AAHNG
AAIAL
AAJBT
AAJKR
AANXM
AANZL
AAPKM
AARHV
AARTL
AASML
AATNV
AATVU
AAUYE
AAWCG
AAYIU
AAYQN
AAYTO
AAYZH
AAZMS
ABAKF
ABBRH
ABDBE
ABDZT
ABECU
ABFSG
ABFTV
ABJNI
ABJOX
ABKCH
ABMQK
ABMXE
ABPLI
ABQBU
ABRTQ
ABSXP
ABTEG
ABTHY
ABTKH
ABTMW
ABULA
ABXPI
ACAOD
ACBXY
ACDTI
ACGFS
ACHSB
ACHXU
ACKNC
ACMDZ
ACMLO
ACOKC
ACPIV
ACPRK
ACSTC
ACZOJ
ADBBV
ADHHG
ADHIR
ADKNI
ADKPE
ADRFC
ADTPH
ADURQ
ADYFF
ADZKW
AEBTG
AEFQL
AEGNC
AEJHL
AEJRE
AEMSY
AENEX
AEOHA
AEPYU
AESKC
AETCA
AEVLU
AEXYK
AEZWR
AFBBN
AFDZB
AFHIU
AFLOW
AFOHR
AFQWF
AFRAH
AFWTZ
AFZKB
AGAYW
AGDGC
AGJBK
AGMZJ
AGQEE
AGQMX
AGRTI
AGWZB
AGYKE
AHAVH
AHBYD
AHKAY
AHPBZ
AHSBF
AHWEU
AHYZX
AIAKS
AIGIU
AIIXL
AILAN
AITGF
AIXLP
AJBLW
AJRNO
AJZVZ
AKMHD
ALMA_UNASSIGNED_HOLDINGS
AMKLP
AMXSW
AMYLF
AMYQR
ANMIH
AOCGG
ARMRJ
ATHPR
AXYYD
AYCSE
AYFIA
BAWUL
BGNMA
CAG
CGR
COF
CSCUP
CUY
CVF
DDRTE
DNIVK
DPUIP
E3Z
EBLON
EBS
ECM
EIF
EIOEI
EJD
EN4
ESBYG
F5P
FERAY
FFXSO
FIGPU
FINBP
FNLPD
FRRFC
FSGXE
FYJPI
GGCAI
GGRSB
GJIRD
GQ7
H13
HF~
HMJXF
HRMNR
HZ~
IAO
IHR
IKXTQ
INH
ITC
IWAJR
IXD
J-C
JBSCW
JZLTJ
KOV
LLZTM
M4Y
NPM
NPVJJ
NQJWS
NU0
O9-
O93
O9I
O9J
OK1
OVT
P6G
PF-
PT4
R9I
ROL
RSV
S1Z
S27
S3A
S3B
SBL
SHX
SISQX
SJYHP
SNE
SNPRN
SNX
SOHCF
SOJ
SPISZ
SRMVM
SSLCW
SSXJD
STPWE
T13
TR2
TSG
U2A
U9L
UG4
UOJIU
UTJUX
UZXMN
VC2
VFIZW
W48
WK8
XSB
Y2W
ZMTXR
ZOVNA
~A9
7X8
ID FETCH-LOGICAL-p209t-a0d076bf39aeb5d30ac71cb86cd9a8efcca068ef0c8222a807ac8eec10637bd43
ISSN 1234-1983
IngestDate Tue Aug 05 09:32:16 EDT 2025
Mon Jul 21 05:53:41 EDT 2025
IsPeerReviewed true
IsScholarly true
Issue 1
Language English
LinkModel OpenURL
MergedId FETCHMERGED-LOGICAL-p209t-a0d076bf39aeb5d30ac71cb86cd9a8efcca068ef0c8222a807ac8eec10637bd43
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
PMID 18263969
PQID 70279752
PQPubID 23479
ParticipantIDs proquest_miscellaneous_70279752
pubmed_primary_18263969
PublicationCentury 2000
PublicationDate 2008-00-00
20080101
PublicationDateYYYYMMDD 2008-01-01
PublicationDate_xml – year: 2008
  text: 2008-00-00
PublicationDecade 2000
PublicationPlace England
PublicationPlace_xml – name: England
PublicationTitle Journal of applied genetics
PublicationTitleAlternate J Appl Genet
PublicationYear 2008
References 24196149 - Theor Appl Genet. 1993 Feb;85(8):985-93
24186250 - Theor Appl Genet. 1994 Sep;88(8):973-80
14564048 - J Appl Genet. 2001;42(2):139-44
12582505 - Theor Appl Genet. 2002 Sep;105(4):564-567
16344114 - Int Rev Cytol. 2005;247:223-84
16453130 - Theor Appl Genet. 2006 May;112(7):1232-8
8106085 - Plant J. 1993 Aug;4(2):403-10
References_xml – reference: 14564048 - J Appl Genet. 2001;42(2):139-44
– reference: 8106085 - Plant J. 1993 Aug;4(2):403-10
– reference: 12582505 - Theor Appl Genet. 2002 Sep;105(4):564-567
– reference: 16344114 - Int Rev Cytol. 2005;247:223-84
– reference: 24196149 - Theor Appl Genet. 1993 Feb;85(8):985-93
– reference: 24186250 - Theor Appl Genet. 1994 Sep;88(8):973-80
– reference: 16453130 - Theor Appl Genet. 2006 May;112(7):1232-8
SSID ssj0038472
Score 1.7438529
Snippet The Ns gene confers resistance of potato to Potato virus S (PVS). Sixteen German and Dutch potato cultivars, all registered in Poland, were found to be...
SourceID proquest
pubmed
SourceType Aggregation Database
Index Database
StartPage 45
SubjectTerms Animals
Deoxyribonucleases, Type II Site-Specific - genetics
False Positive Reactions
Genetic Markers
Genetic Predisposition to Disease
Lycopersicon esculentum - genetics
Lycopersicon esculentum - virology
Plant Diseases - genetics
Plant Diseases - virology
Potyvirus - genetics
Potyvirus - pathogenicity
Random Amplified Polymorphic DNA Technique
Solanum tuberosum - genetics
Solanum tuberosum - virology
Title Endonuclease restriction of SCAR amplicons SC811 is required to identify Ns-false-positive markers in PVS-susceptible potato cultivars
URI https://www.ncbi.nlm.nih.gov/pubmed/18263969
https://www.proquest.com/docview/70279752
Volume 49
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9NAEF6FcoED4k157oGeokVrr5_HEBVVSPSAG1T1Eq3X69YE7Mh2WsU_gB_CL2XGa5ukoRJwcSJ7tbY8n2Zmvd98Q8hbVEFLhYcNiJ2QOa6tWeC5mmmIpo4PAT-MsVD407F3NHM-nrqno9HPDdbSqo7fqeaPdSX_Y1U4B3bFKtl_sOwwKZyA_2BfOIKF4fhXNj7EVhwoSAyhaIxNNspM9RlgNJ18HsuWL45smGgaWBZ2Ly81cn8hzYSkM2urdNP1-LhiKTytZobDdYms1nKBtb0Z-MgvEatWVct_wTqrZVFjwxjU7MguZbcbtJvfyi6_hafHQskhd48a-XVRdEwOWTbr_DdZ6KJsZF5cVQtpKP0Qwg-m7kFgy6thUFSXjf6mmvWCna1N7gvQXdXDgDN5jtv_70XZzdOGVrn1fWPTGdvCYVZoGt303toInG6h0rheo0q5ExH4wHO3Qtc2qp4b0Fh-b7GBqywRmqYx1_S3-0u3yG0bliLYJWNmT_poLyC6tzvq_bNuS-B2N0Vp2m6am1cybUZzcp_c60xFJwZXD8hI5w_J3cl52cmx6EfkxybC6AbCaJFSRBgdEEZbhNGsoj3CaF3QHmF0B2G0QxjNcnoNYdQgjA4Ie0xmHw5Ppkes69zBljYPayZ5wn0vTkUodewmgkvlWyoOPJWEMtApuA3uwS9XmJ_KgPtSBVorCxJmP04c8YTs5UWunxGqsTe2ZytL4ZcEwWEYT4QnQ53GWopgn7zp3-ccPCNud8lcF6tq7nPbD8Hd7JOn5jXPl0bAZd7b4vmNV16QO4YYhN_aXpK9ulzpV5B91vHr1vy_ACC9inw
linkProvider Springer Nature
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Endonuclease+restriction+of+SCAR+amplicons+SC811+is+required+to+identify+Ns-false-positive+markers+in+PVS-susceptible+potato+cultivars&rft.jtitle=Journal+of+applied+genetics&rft.au=Szajko%2C+Katarzyna&rft.au=Chrzanowska%2C+Miros%C5%82awa&rft.au=Strzelczyk-Zyta%2C+Danuta&rft.au=Zag%C3%B3rska%2C+Helena&rft.date=2008&rft.issn=1234-1983&rft.volume=49&rft.issue=1&rft.spage=45&rft_id=info:doi/10.1007%2FBF03195248&rft_id=info%3Apmid%2F18263969&rft.externalDocID=18263969
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1234-1983&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1234-1983&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1234-1983&client=summon