Simultaneous determination of cefepime and L-arginine by micellar electrokinetic chromatography and applications to commercial injections
A simple micellar electrokinetic chromatography (MEKC) with UV detection is described for simultaneous analysis of cefepime and L-arginine. The determination of cefepime and L-arginine in pharmaceutical preparations was performed at 25degreesC using a background electrolyte consisting of Tris buffer...
Saved in:
Published in | Journal of separation science Vol. 28; no. 16; pp. 2173 - 2179 |
---|---|
Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Germany
01.11.2005
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | A simple micellar electrokinetic chromatography (MEKC) with UV detection is described for simultaneous analysis of cefepime and L-arginine. The determination of cefepime and L-arginine in pharmaceutical preparations was performed at 25degreesC using a background electrolyte consisting of Tris buffer with sodium dodecyl sulfate (SDS) as the electrolyte solution. Several parameters affecting the separation of the drugs were studied, including the pH and concentrations of the Tris buffer and SDS. Under optimal MEKC conditions, good separation with high efficiency and short analysis times is achieved. Using cefazolin as an internal standard, the linear ranges of the method for the determination of cefepime and L-arginine were over 5-100 microg/mL; the detection limits of cefepime (signal to noise ratio = 3; injection 3.45 kPa, 3 s) and L-arginine (signal to noise ratio = 3; injection 3.45 kPa, 3 s) were 2 microg/mL and 4 microg/ mL, respectively. Applicability of the proposed method for the determination of cefepime and L-arginine in commercial injections was demonstrated. |
---|---|
AbstractList | A simple micellar electrokinetic chromatography (MEKC) with UV detection is described for simultaneous analysis of cefepime and L-arginine. The determination of cefepime and L-arginine in pharmaceutical preparations was performed at 25degreesC using a background electrolyte consisting of Tris buffer with sodium dodecyl sulfate (SDS) as the electrolyte solution. Several parameters affecting the separation of the drugs were studied, including the pH and concentrations of the Tris buffer and SDS. Under optimal MEKC conditions, good separation with high efficiency and short analysis times is achieved. Using cefazolin as an internal standard, the linear ranges of the method for the determination of cefepime and L-arginine were over 5-100 microg/mL; the detection limits of cefepime (signal to noise ratio = 3; injection 3.45 kPa, 3 s) and L-arginine (signal to noise ratio = 3; injection 3.45 kPa, 3 s) were 2 microg/mL and 4 microg/ mL, respectively. Applicability of the proposed method for the determination of cefepime and L-arginine in commercial injections was demonstrated. |
Author | Wu, Hsin-Lung Chen, Yi-Ru Lin, Shun-Jin Chen, Su-Hwei Chou, Yu-Wei |
Author_xml | – sequence: 1 givenname: Yi-Ru surname: Chen fullname: Chen, Yi-Ru organization: Graduate Institute of Pharmaceutical Sciences, Kaohsiung Medical University, Kaohsiung, Taiwan – sequence: 2 givenname: Shun-Jin surname: Lin fullname: Lin, Shun-Jin – sequence: 3 givenname: Yu-Wei surname: Chou fullname: Chou, Yu-Wei – sequence: 4 givenname: Hsin-Lung surname: Wu fullname: Wu, Hsin-Lung – sequence: 5 givenname: Su-Hwei surname: Chen fullname: Chen, Su-Hwei |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/16318214$$D View this record in MEDLINE/PubMed |
BookMark | eNo1UDlPwzAY9VBED1gZkSe2gO1c9ogqLqkSAzBHjv2ldYntYDtDfwL_mhLK9KR3Se8t0cx5BwhdUXJLCWF3-xjVLSOkJIRwOkMLWtEyEzmp5mgZ454QWnNBztGcVjnljBYL9P1m7Ngn6cCPEWtIEKxxMhnvsO-wgg4GYwFLp_Emk2FrnHGA2wO2RkHfy4ChB5WC_zzyySisdsFbmfw2yGF3mIJyGHqjptKIk8fKWwtBGdlj4_bH9K9wgc462Ue4POEKfTw-vK-fs83r08v6fpMNjIiUMQBgQueiy6kqlBCKs5IXVQGacl3UnGnNZNeytqNlW3d1rXNJoRK0kFQUeb5CN3-9Q_BfI8TUWBOnKdMHTcV5wcqaHo3XJ-PYWtDNEIyV4dD8n5f_ANgfdWM |
ContentType | Journal Article |
DBID | CGR CUY CVF ECM EIF NPM 7X8 |
DOI | 10.1002/jssc.200500081 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
DatabaseTitleList | MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Engineering Chemistry |
EndPage | 2179 |
ExternalDocumentID | 16318214 |
Genre | Research Support, Non-U.S. Gov't Journal Article |
GroupedDBID | --- .3N .GA .Y3 05W 0R~ 10A 1L6 1OC 31~ 33P 3SF 3WU 4.4 4ZD 50Y 50Z 51W 51X 52M 52N 52O 52P 52S 52T 52U 52W 52X 53G 5GY 5VS 66C 702 7PT 8-0 8-1 8-3 8-4 8-5 8UM 930 A03 AAESR AAEVG AAHBH AAHHS AANLZ AAONW AASGY AAXRX AAZKR ABCUV ABDBF ABIJN ABJNI ABPVW ACAHQ ACBWZ ACCFJ ACCZN ACGFS ACIWK ACPOU ACXBN ACXQS ADBBV ADEOM ADIZJ ADKYN ADMGS ADOZA ADXAS ADZMN AEEZP AEIGN AEIMD AENEX AEQDE AEUQT AEUYR AFBPY AFFPM AFGKR AFPWT AFZJQ AHBTC AI. AITYG AIURR AIWBW AJBDE AJXKR ALAGY ALMA_UNASSIGNED_HOLDINGS ALUQN AMBMR AMYDB ATUGU AUFTA AZBYB AZFZN AZVAB BAFTC BDRZF BFHJK BHBCM BMNLL BMXJE BNHUX BROTX BRXPI BY8 CGR CS3 CUY CVF D-E D-F DCZOG DPXWK DR2 DRFUL DRSTM DU5 EBD ECM EIF EJD F00 F01 F04 F5P FEDTE G-S G.N GNP GODZA H.T H.X HGLYW HHZ HVGLF HZ~ IX1 J0M JPC KQQ LATKE LAW LC2 LC3 LEEKS LH4 LITHE LOXES LP6 LP7 LUTES LW6 LYRES MEWTI MK4 MRFUL MRSTM MSFUL MSSTM MXFUL MXSTM N04 N05 N9A NF~ NNB NPM O66 O9- OIG P2P P2W P2X P4D PQQKQ Q.N Q11 QB0 QRW R.K RNS ROL RWI RX1 RYL SUPJJ TUS UB1 UPT VH1 W8V W99 WBFHL WBKPD WIH WIK WJL WOHZO WXSBR WYISQ XG1 XPP XV2 YQT ~IA ~KM ~WT 7X8 |
ID | FETCH-LOGICAL-p209t-2eee29d39f31c4c99c8258464ed18d4782dd2afb2bf15b7f77d3a1e6914a19433 |
ISSN | 1615-9306 |
IngestDate | Fri Aug 16 09:49:11 EDT 2024 Sat Sep 28 07:50:26 EDT 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 16 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-p209t-2eee29d39f31c4c99c8258464ed18d4782dd2afb2bf15b7f77d3a1e6914a19433 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
PMID | 16318214 |
PQID | 68842571 |
PQPubID | 23479 |
PageCount | 7 |
ParticipantIDs | proquest_miscellaneous_68842571 pubmed_primary_16318214 |
PublicationCentury | 2000 |
PublicationDate | 2005-Nov 20051101 |
PublicationDateYYYYMMDD | 2005-11-01 |
PublicationDate_xml | – month: 11 year: 2005 text: 2005-Nov |
PublicationDecade | 2000 |
PublicationPlace | Germany |
PublicationPlace_xml | – name: Germany |
PublicationTitle | Journal of separation science |
PublicationTitleAlternate | J Sep Sci |
PublicationYear | 2005 |
SSID | ssj0017890 |
Score | 1.8352069 |
Snippet | A simple micellar electrokinetic chromatography (MEKC) with UV detection is described for simultaneous analysis of cefepime and L-arginine. The determination... |
SourceID | proquest pubmed |
SourceType | Aggregation Database Index Database |
StartPage | 2173 |
SubjectTerms | Arginine - analysis Arginine - chemistry Cefazolin - chemistry Cephalosporins - analysis Cephalosporins - chemistry Chromatography, Micellar Electrokinetic Capillary - standards Hydrogen-Ion Concentration Reference Standards Sodium Dodecyl Sulfate Tromethamine |
Title | Simultaneous determination of cefepime and L-arginine by micellar electrokinetic chromatography and applications to commercial injections |
URI | https://www.ncbi.nlm.nih.gov/pubmed/16318214 https://search.proquest.com/docview/68842571 |
Volume | 28 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwELaWcoAeEJRHy9MHrobEceL4iEqrVbUUqd0VyylKYpum0GS1mxzgxpF_zdiJN4ko4nGJEsuJEs8nz-d45huEXvqUa5ObR3gQ5YSJSBEBVJ9oEUSRipnkttbhu9NoumAny3A5mXwfZpfU2av827V5Jf9jVWgDu5os2X-w7Pah0ADnYF84goXh-Fc2Pi9MPGBaKhPHKl1gi-OAudJqVVy1-wMzkq4_mWIQyhBOU4P-i4k_7argfIZ2o9yaX6wroLCdjHUr5DrY4TZEFb7jylRpsmIdlzaQq_vh9yvF3ahWWRzep_O0fTRBO919LMhZsw0KavUMzi-akpwUZd-3amzfhnxQxdaL2LbppijJrOncr_t7EXZpfP2EC4yKiMCLhjMyjYfIG8-vbeGTzlfDpbjWD7S6spebjVWpDC3z6T2e2-U_fZ8cL2azZH60nN9ANykXoYkKfXu2VSDzTZ6wWbO713S6nx59PX7671colqnM76I73fjjNy1e7qGJKvfQrUNX2W8P7Q5EKO-jH0MU4RGKcKWxQxEGMOAeRTj7ih2K8BhFeIwie-MQRbiucI8i3KPoAVocH80Pp6Qr0EFW1BM1oUopKmQgdODnLBcij6khtExJP5YMyKeUNNUZzbQfZlxzLoPUV5HwWeoLFgQP0U5ZlWof4ZhTnXkyVCmXzNMZTBpM59Bbph5XIjpAL9zwJjBa9vPsuCRRbHaSuX-AHrWjnqxanZYElhqwevbZ4z_e-wTd7sH5FO3U60Y9A7JZZ88tHH4CFbiJOQ |
link.rule.ids | 315,786,790,27955,27956 |
linkProvider | Wiley-Blackwell |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Simultaneous+determination+of+cefepime+and+L-arginine+by+micellar+electrokinetic+chromatography+and+applications+to+commercial+injections&rft.jtitle=Journal+of+separation+science&rft.au=Chen%2C+Yi-Ru&rft.au=Lin%2C+Shun-Jin&rft.au=Chou%2C+Yu-Wei&rft.au=Wu%2C+Hsin-Lung&rft.date=2005-11-01&rft.issn=1615-9306&rft.volume=28&rft.issue=16&rft.spage=2173&rft.epage=2179&rft_id=info:doi/10.1002%2Fjssc.200500081&rft.externalDBID=NO_FULL_TEXT |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1615-9306&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1615-9306&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1615-9306&client=summon |