Thrombin responses in human endothelial cells. Contributions from receptors other than PAR1 include the transactivation of PAR2 by thrombin-cleaved PAR1

• Published in: The Journal of biological chemistry Vol. 275; no. 18; p. 13502
• Main Authors: O'Brien, P J, Prevost, N, Molino, M, Hollinger, M K, Woolkalis, M J, Woulfe, D S, Brass, L F
• Format: Journal Article
• Language: English
• Published: United States 05.05.2000
• Subjects: Cells, Cultured; Endothelium, Vascular - metabolism; Humans; Receptor, PAR-1; Receptors, Cell Surface - metabolism; Receptors, Thrombin - metabolism; Signal Transduction - drug effects; Thrombin - metabolism; Thrombin - pharmacology; Transcriptional Activation - drug effects

The recent identification of two new thrombin receptors, PAR3 and PAR4, led us to re-examine the basis for endothelial cell responses to thrombin. Human umbilical vein endothelial cells (HUVEC) are known to express PAR1 and the trypsin/tryptase receptor, PAR2. Northern blots detected both of those r...