Mediation of proliferating cell nuclear antigen (PCNA)-dependent DNA replication through a conserved p21(Cip1)-like PCNA-binding motif present in the third subunit of human DNA polymerase delta

• Published in: The Journal of biological chemistry Vol. 276; no. 52; p. 49258
• Main Authors: Ducoux, M, Urbach, S, Baldacci, G, Hübscher, U, Koundrioukoff, S, Christensen, J, Hughes, P
• Format: Journal Article
• Language: English
• Published: United States 28.12.2001
• Subjects: Amino Acid Motifs; Amino Acid Sequence; Animals; Cyclin-Dependent Kinase Inhibitor p21; Cyclins - genetics; Cyclins - metabolism; DNA Polymerase III - chemistry; DNA Polymerase III - metabolism; DNA Replication - physiology; HeLa Cells; Humans; Immunohistochemistry; Molecular Sequence Data; Mutation; Peptide Fragments - metabolism; Phosphorylation; Proliferating Cell Nuclear Antigen - genetics; Proliferating Cell Nuclear Antigen - metabolism; Protein Binding; Protein Subunits; Recombinant Proteins - chemistry; Recombinant Proteins - metabolism; Sequence Alignment

The subunit that mediates binding of proliferating cell nuclear antigen (PCNA) to human DNA polymerase delta has not been clearly defined. We show that the third subunit of human DNA polymerase delta, p66, interacts with PCNA through a canonical PCNA-binding sequence located in its C terminus. Conve...