Role of p53 in cell cycle regulation and apoptosis following exposure to proteasome inhibitors

• Published in: Cell growth & differentiation Vol. 11; no. 5; pp. 239 - 246
• Main Authors: Chen, F, Chang, D, Goh, M, Klibanov, S A, Ljungman, M
• Format: Journal Article
• Language: English
• Published: United States 01.05.2000
• Subjects: Acetylcysteine - analogs & derivatives; Acetylcysteine - pharmacology; Apoptosis - drug effects; Apoptosis - physiology; Cell Nucleus - metabolism; Colonic Neoplasms; Cyclin-Dependent Kinase Inhibitor p21; Cyclins - analysis; Cyclins - metabolism; Cysteine Endopeptidases - metabolism; Cysteine Proteinase Inhibitors - pharmacology; Fibroblasts - cytology; Fibroblasts - enzymology; G1 Phase - drug effects; G1 Phase - physiology; G2 Phase - drug effects; G2 Phase - physiology; Humans; Interphase - drug effects; Interphase - physiology; Mitosis - drug effects; Mitosis - physiology; Multienzyme Complexes - metabolism; Proteasome Endopeptidase Complex; Skin - cytology; Tumor Cells, Cultured; Tumor Suppressor Protein p53 - physiology

In this study, we explored what effect inhibitors of the 26S proteasome have on cell cycle distribution and induction of apoptosis in human skin fibroblasts and colon cancer cells differing in their p53 status. We found that proteasome inhibition resulted in nuclear accumulation of p53. This was sur...