Involvement of cyclin dependent kinase 5 and its activator p35 in staurosporine-induced apoptosis of cortical neurons

• Published in: Acta pharmacologica Sinica Vol. 25; no. 9; p. 1105
• Main Authors: Zhang, Bai-Fang, Peng, Fang-Fang, Zhang, Wen, Shen, Han, Wu, Shao-Bo, Wu, Dong-Cheng
• Format: Journal Article
• Language: English
• Published: United States 01.09.2004
• Subjects: Animals; Apoptosis - drug effects; Cells, Cultured; Cerebral Cortex - cytology; Cerebral Cortex - metabolism; Cyclin-Dependent Kinase 4; Cyclin-Dependent Kinase 5; Cyclin-Dependent Kinases - metabolism; DNA Fragmentation; Embryo, Mammalian; Mice; Nerve Tissue Proteins - metabolism; Neurons - cytology; Neurons - metabolism; Phosphotransferases - metabolism; Proto-Oncogene Proteins - metabolism; Staurosporine - pharmacology; Tumor Suppressor Protein p53 - metabolism

To investigate whether cyclin-dependent kinase 5 and its regulatory protein p35 was involved in staurosporine-induced apoptosis of cortical neuronal cultures. Primary cerebral cortical neurons were exposed to 300 nmol/L staurosporine. After incubation for different time, morphological alterations we...