The effect of digitalis glycosides-specific antisera on the binding of the glycosides to (Na+ + K+)-ATPase

The capability of digitalis glycoside-specific antisera to reverse the glycoside-induced inhibition of (Na+ + K+)-ATPase activity was examined. The antisera-induced reversal of the ATPase inhibition caused by digoxin was considerably slower (k-1 = 0.1177 . 10-3 .S-1) than the reversal caused by dihy...

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Bibliographic Details
Published inArzneimittel-Forschung Vol. 31; no. 4; p. 625
Main Author Bossaller, C
Format Journal Article
LanguageEnglish
Published Germany 1981
Subjects
Animals
Cattle
Digitalis Glycosides - immunology
Digitalis Glycosides - metabolism
Humans
Immune Sera
Myocardium - enzymology
Protein Binding - drug effects
Sodium-Potassium-Exchanging ATPase - antagonists & inhibitors
Sodium-Potassium-Exchanging ATPase - metabolism
Time Factors
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Summary:The capability of digitalis glycoside-specific antisera to reverse the glycoside-induced inhibition of (Na+ + K+)-ATPase activity was examined. The antisera-induced reversal of the ATPase inhibition caused by digoxin was considerably slower (k-1 = 0.1177 . 10-3 .S-1) than the reversal caused by dihydrodigoxin and dihydrodigitoxin (k-1 = 4.462 . 10-3 . S-1 and K-1 = 3.609 .10-3 . S-1, respectively). In addition, the dissociation rate constants of the ouabain-induced dissociation of the various glycosides from the glycoside-ATPase complex were determined: The dissociation rate constants of the antisera-induced restoration of enzyme activity and that of the ouabain-induced dissociation of glycosides from the glycoside-enzyme complex were not significantly different.
ISSN:0004-4172